紫山药多糖对D-半乳糖衰老模型大鼠肝、脑的影响

发布时间：2018-07-08 16:59

本文选题：紫山药多糖 + 衰老　；参考：《食品科学》2017年13期

【摘要】：目的:探讨紫山药多糖对D-半乳糖所致衰老模型大鼠的抗衰老作用及其作用机理。方法:以D-半乳糖建立大鼠衰老模型,灌胃紫山药多糖(20、100、500 mg/(kg·d))45 d后,检测大鼠肝、脑组织中总抗氧化能力(total antioxidant capacity,T-AOC)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力,检测谷胱甘肽(glutathione,GSH)及过氧化终产物丙二醛(malondialdehyde,MDA)含量,应用免疫印迹实验评价肝、脑中p53、p21蛋白的表达。结果:100 mg/(kg·d)紫山药多糖可显著提高D-半乳糖衰老模型大鼠肝、脑中T-AOC、GSH-Px活力、GSH含量,降低过氧化产物MDA含量,抑制衰老基因p53、p21的蛋白表达。结论:紫山药多糖具有显著的抗大鼠肝、脑衰老损伤的作用,作用机理可能与p53/p21信号通路有关。
[Abstract]:Aim: to investigate the anti-aging effect and mechanism of Zyam polysaccharide on D-galactose induced aging model rats. Methods: the aging model of rats was established by D-galactose. The activities of glutathione peroxidase (GSH-Px) and glutathione peroxidase (GSH-Px) in the liver and brain tissues of rats were measured after oral administration of polysaccharides of purple yam (20100500 mg/ (kg d) for 45 days). The contents of glutathione (GSH) and malondialdehyde (MDA) were measured. The expression of p53 p21 in liver and brain was evaluated by Western blot. Results the content of GSH in liver and brain of rat model of aging induced by D-galactose was significantly increased, the content of peroxidation product was decreased, and the protein expression of senescence gene p53 / p21 was inhibited by 0: 100 mg/ (kg d) purple yam polysaccharide. Conclusion: Zyam polysaccharide has a significant anti-aging effect on rat liver and brain, and the mechanism may be related to the p53/p21 signaling pathway.
【作者单位】：中国农业大学食品科学与营养工程学院植物源功能食品北京市重点实验室;新时代健康产业(集团)有限公司;国家蔬菜工程技术研究中心;
【基金】：北京市农林科学院科技创新能力建设专项(KJCX20140111;KJCX20150201)
【分类号】：R285.5;R-332

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