母婴分离应激对rd新生小鼠行为的影响

发布时间：2018-07-10 02:01

本文选题：rd小鼠 + 母婴分离　；参考：《中国比较医学杂志》2017年04期

【摘要】：目的 rd小鼠是一种出生1周左右自发致盲的遗传性视网膜变性小鼠。通过母婴分离应激实验,检测其对rd新生小鼠的焦虑样和抑郁样行为的影响,初步评价rd小鼠能否成为眼部疾病与焦虑抑郁研究新的模型。方法将出生第1天的rd小鼠分为母婴分离组(n=9)和对照组(n=9)。母婴分离组每天与母鼠分离4 h,共分离28 d。从第29天开始行为测试实验,通过Smart3动物行为跟踪软件检测母婴分离组和对照组在旷场实验和十字高架实验中的焦虑样行为,在强迫游泳实验和悬尾实验中的抑郁样行为。结果在旷场实验中,母婴分离组在旷场中心的时间比例(0.88%)和运动距离(28.17±5.65)cm均明显少于对照组(2.61%,109.9±9.79 cm;P=0.04,P=0.001);在十字高架实验中,母婴分离组在开放臂的时间(40.64±4.13)s明显少于对照组(91.73±11.26 s,P0.01);在强迫游泳实验中,母婴分离组的不动时间(126.5±10.22)s明显多于对照组(77.75±16.83s,P=0.02);在悬尾实验中,母婴分离组的不动时间(21.56±6.83)s多于对照组(7.37±3.22 s,P=0.03)。结论rd小鼠母婴分离28 d应激,能够明显增加rd小鼠的焦虑样行为和抑郁样行为。
[Abstract]:Objective Rd mouse is a kind of hereditary retinal degeneration mice born about 1 week after birth. In order to evaluate whether Rd mice can become a new model of ocular diseases and anxiety and depression, the effects of the stress on Rd newborn mice were tested by the stress test of mother and child separation, and the effects of Rd on anxiety and depressive behavior in Rd newborn mice were preliminarily evaluated. Methods Rd mice on the first day of birth were divided into two groups: maternal and infant separation group (nong9) and control group (nong9). The mother and infant group were separated from the mother for 4 hours and separated for 28 days. From the 29th day, Smart3 animal behavior tracking software was used to detect anxiety behavior in open field test and cross elevated test, depression behavior in forced swimming test and tail suspension test. Results in the open field experiment, the time ratio (0.88%) and the distance of exercise (28.17 卤5.65) cm in the mother-infant separation group were significantly lower than those in the control group (2.61 卤9.79 cm ~ (-1) P ~ (0.04) P ~ (0.001). In the forced swimming experiment, the immobility time in the mother-infant separation group (126.5 卤10.22) s was significantly longer than that in the control group (77.75 卤16.83 s), and in the tail suspension test, the immobility time of the mother and infant separation group (21.56 卤6.83) s was higher than that of the control group (7.37 卤3.22 sP0.03). Conclusion stress for 28 days in Rd mice can significantly increase anxiety and depressive behavior in Rd mice.
【作者单位】：内蒙古医科大学药学院;内蒙古医科大学分子病理学重点实验室;沈阳药科大学生命科学与生物制药学院;
【基金】：国家自然科学基金(编号31260260) 内蒙古医科大学博士启动基金(编号:nybq2010zcl)
【分类号】：R-332;R749

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