骨碎补总黄酮对免疫低下模型小鼠免疫功能的影响

发布时间：2018-07-10 02:29

本文选题：骨碎补总黄酮 + 免疫低下　；参考：《天然产物研究与开发》2016年03期

【摘要】：本文旨在研究骨碎补总黄酮(Rhizoma Drynariae Flavonoids,RDF)对环磷酰胺(CTX)诱导的免疫低下模型小鼠免疫功能的影响。通过腹腔注射CTX建立免疫低下小鼠模型,全自动生化仪检测小鼠血中白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)、淋巴细胞百分率(LYNPH%)计数,ELISA法检测血清IL-2、TNF-α、IFN-γ水平,MTT法检测脾淋巴细胞的增殖反应,流式细胞仪检测外周血T淋巴细胞亚群CD4~+、CD8~+水平,RTPCR检测脾组织T-bet、GATA-3 mRNA的表达。并对免疫器官指数,吞噬细胞吞噬功能,脾脏乳酸脱氢酶(LDH)和酸性磷酸酶(ACP)活性,血清溶血素水平和迟发型超敏反应等进行检测。结果显示,与模型组比较,RDF可明显提高小鼠的免疫器官指数,廓清指数K和吞噬指数α,WBC、RBC、HGB、LYNPH%,IL-2、TNF-α、IFN-γ水平,脾脏LDH和ACP活性,血清溶血素,耳肿胀度,CD4~+、CD4~+/CD8~+比值和脾组织T-bet mRNA的表达(P0.05),而CD8~+T细胞数,GATA-3 mRNA的相对表达量明显降低(P0.05)。研究结果表明,RDF能够提高CTX所致的免疫低下模型小鼠的非特异性免疫、体液免疫和细胞免疫功能,对小鼠的免疫功能有正向调节作用。
[Abstract]:The aim of this study was to study the effect of Rhizoma Drynariae Flavonoidsl RDF (RDF) on immune function in immunocompromised mice induced by cyclophosphamide (CTX). The mice model of immunosuppression was established by intraperitoneal injection of CTX. WBC, RBC, HGB and LYNPH% were measured by automatic biochemical instrument. Elisa was used to detect the levels of IL-2TNF- 伪 and IFN- 纬 in serum and the proliferation of splenic lymphocytes was detected by MTT assay. The expression of T-beta-GATA-3 mRNA in splenic tissue was detected by RT-PCR. The index of immune organs, phagocytic function of phagocytes, activities of lactic dehydrogenase (LDH) and acid phosphatase (ACP) in spleen, serum hemolysin level and delayed type hypersensitivity were determined. The results showed that RDF could significantly improve the immune organ index, clearance index K and phagocytosis index of RDF in mice compared with the model group, and the levels of IL-2TNF- 伪 -IFN- 纬, spleen LDH and ACP activities, serum hemolysin level, and phagocytic index of RDF were significantly increased. The ratio of CD4 ~ / CD4 ~ / CD8 ~ and T-bet mRNA expression in spleen were significantly decreased (P0.05), while the relative expression of GATA-3 mRNA in CD8 ~ T cells was significantly decreased (P0.05). The results showed that RDF could improve the immune function of CTX-induced hypoimmune mice, including nonspecific immunity, humoral immunity and cellular immunity, and had a positive effect on the immune function of mice.
【作者单位】：广西壮族自治区妇幼保健院;广西医科大学;广西医科大学第一附属医院;
【基金】：广西自然科学基金(2014GXNSFAA118185) 广西科技基础条件平台建设项目(10-108-19)
【分类号】：R285.5;R-332

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