兔颈动脉粥样硬化狭窄模型的建立及天冬酰胺内肽酶的表达

发布时间：2018-07-11 16:36

本文选题：动脉粥样硬化 + 颈动脉狭窄　；参考：《中国脑血管病杂志》2016年03期

【摘要】：目的探讨兔颈动脉粥样硬化(AS)狭窄模型中天冬酰胺内肽酶(AEP)的表达。方法将14只新西兰大白兔按随机数字表法分成模型组(8只)和假手术组(6只),模型组通过手术损伤颈动脉内膜。术后两组均给予含镁高脂饲料饲养兔10周,每2周进行体质量称量及血脂检验,术后第5周和第10周末均行MRI观察斑块形成及管腔狭窄情况,术后第10周末收集标本后切片,苏木素-伊红(HE)染色观察病理变化,免疫组化染色分析AEP表达水平。结果模型组1只兔损伤颈动脉死亡。高脂饲养后,两组兔体质量、血脂水平均较术前升高(P0.01)。模型组术后第5周和第10周末两次MRI示管腔狭窄率分别为(16±11)%和(53±20)%,组内差异有统计学意义(t=-4.83,P0.01);假手术组两次MRI均未见管腔狭窄。模型组HE染色可见内膜增生,AS斑块形成,斑块内脂质沉积,巨噬细胞、平滑肌细胞迁移浸润形成泡沫细胞;假手术组未见AS形成。AEP在模型组兔颈动脉组织中高表达,在假手术组中很少表达,吸光度值分别为0.072 0±0.028 0和0.002 0±0.000 9,差异有统计学意义(t=6.61,P0.01)。结论通过损伤颈动脉内膜联合含镁高脂饲料饲养的方法可以确切、可靠、迅速地建立兔颈动脉AS狭窄模型;AEP在颈动脉AS性狭窄病变中呈高表达,可能参与了AS斑块的形成。
[Abstract]:Objective to investigate the expression of asparagine endopeptidase (AEP) in rabbit carotid atherosclerosis (as) stenosis model. Methods 14 New Zealand rabbits were randomly divided into two groups: model group (n = 8) and sham-operated group (n = 6). The rabbits were fed with high fat diet containing magnesium for 10 weeks. The body weight and blood lipid were measured every 2 weeks. The plaque formation and lumen stenosis were observed by MRI at the 5th and 10th week after operation. The specimens were collected and sectioned at the 10th week after operation. The pathological changes were observed by hematoxylin-eosin (HE) staining and the expression of AEP was analyzed by immunohistochemical staining. Results one rabbit died of carotid artery injury in the model group. After hyperlipidemia feeding, the body mass and blood lipid level of both groups were higher than those before operation (P0.01). In the model group, the stenosis rate on MRI was (16 卤11)% and (53 卤20) at the 5th week and 10th week after operation, respectively, and the difference was statistically significant (t = 4.83, P 0.01), but no stenosis was found on MRI in the sham operation group. In the model group, the formation of atherosclerotic plaques, lipid deposition, migration and infiltration of macrophages and smooth muscle cells into foam cells were observed by HE staining, while in the sham operation group, there was no high expression of AEP in the carotid artery of the model group. In the sham-operation group, the absorbance was 0.072 卤0.028 0 and 0.002 0 卤0.000 9, respectively. The difference was statistically significant (t = 6.61, P0.01). Conclusion the high expression of AEP in carotid atherosclerotic lesions can be established quickly by the method of carotid intima injury combined with magnesium high fat diet, which may be involved in the formation of atherosclerotic plaques.
【作者单位】：上海交通大学医学院附属仁济医院神经外科;上海交通大学医学院附属仁济医院影像科;
【基金】：上海市科学技术委员会资助项目(13140903900)
【分类号】：R743;R-332

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