铁皮石斛花对肾上腺皮质激素致肾阴虚模型小鼠的影响

发布时间：2018-07-13 12:11

【摘要】：目的:探讨铁皮石斛花"滋阴功效"对肾上腺皮质激素致肾阴虚模型小鼠的作用。方法:采用ICR小鼠皮下注射氢化可的松3次造成肾阴虚模型小鼠,连续灌胃给予铁皮石斛花21 d。给药期间观测小鼠体重、摄食量、饮水量;末次给药后检测小鼠面温、抓力、痛阈和自主活动;取血测定血清中丙二醛(MDA)、超氧化物歧化酶(SOD)、环磷腺苷(c AMP)和环磷鸟苷(c GMP)水平。结果:1与正常对照组比较,模型对照组小鼠体重、摄食量、痛阈值、抓力及血清SOD活力明显降低,饮水量、自主活动次数、面温、MDA、c AMP含量及c AMP/c GMP比值明显升高。2与模型对照组比较,铁皮石斛花各剂量均能明显减少模型小鼠自主活动次数,降低面温,升高痛阈值、抓力及血清SOD活力;1.0 g/kg和4.0 g/kg剂量能明显增加摄食量;1.0 g/kg和2.0 g/kg剂量能明显降低饮水量、血清c AMP水平及c AMP/c GMP比值。3铁皮石斛花与茎相比,铁皮石斛花在减少饮水量,升高痛阈值及血清SOD活力方面略优于茎;而铁皮石斛茎在调节环核苷酸系统,降低血清MDA含量方面优于花。结论:铁皮石斛花具有传统滋阴功效,可改善肾阴虚模型小鼠肾上腺皮质功能亢进症状,其作用机制可能与调节机体环核苷酸系统及清除体内自由基有关。
[Abstract]:Objective: to study the effect of Dendrobium candidum flower "nourishing yin" on kidney yin deficiency model induced by adrenocortical hormone. Methods: ICR mice were subcutaneously injected with hydrocortisone for 3 times to make kidney yin deficiency model mice and were given Dendrobium candidum continuously for 21 days. During administration, the body weight, food intake and drinking water were measured. After the last administration, the surface temperature, grasping power, pain threshold and independent activity were measured. The serum levels of malondialdehyde (MDA), superoxide dismutase (SOD), cyclic adenosine monophosphate (c-AMP) and cyclic phosphoguanosine (cGMP) were measured. Results compared with the normal control group, the weight, food intake, pain threshold, grasping power and serum SOD activity of the mice in the model control group were significantly lower than those in the normal control group. Compared with the model control group, each dose of Dendrobium candidum could significantly reduce the number of autonomous activities, decrease the surface temperature, and raise the pain threshold, compared with the model control group, the content of MDA-c-AMP and the ratio of c-AMP / cGMP of Dendrobium officinalis were significantly higher than those of the model control group. The concentration of serum sod activity (1.0 g/kg and 4.0 g/kg) increased significantly (1.0 g/kg and 2.0 g/kg). The levels of serum camp and c-AMP / cGMP ratio of Dendrobium candidum decreased significantly compared with those of stem, and the water intake of Dendrobium candidum decreased significantly compared with that of stem. Raising pain threshold and serum SOD activity were slightly superior to stem, while stem of Dendrobium officinale was superior to flower in regulating cyclic nucleotide system and reducing serum MDA content. Conclusion: Dendrobium candidum has the traditional effect of nourishing yin and can improve the symptoms of adrenal cortex hyperfunction in mice with kidney yin deficiency. The mechanism may be related to regulating the system of cyclic nucleotides and scavenging free radicals in vivo.
【作者单位】：温州医科大学;浙江中医药大学;浙江工业大学;
【基金】：国家自然科学基金(81673638) 国家"十二五"科技支撑计划(2011BAI04B06) 浙江中医药大学校级课题(2015ZZ11,2016ZG16)
【分类号】：R285.5;R-332

【相似文献】