小叶丁香总皂苷对衰老模型小鼠的抗氧化作用研究

发布时间：2018-07-15 12:05

【摘要】：目的:考察小叶丁香总皂苷对D-半乳糖致衰老模型小鼠抗氧化作用。方法:采用小鼠颈背部皮下注射D-半乳糖溶液200 mg·kg-1·d-1建立衰老模型;造模成功后,将小鼠随机分为模型对照组、小叶丁香总皂苷低剂量组(60 mg·kg-1)、小叶丁香总皂苷高剂量组(120 mg·kg-1)和阳性药对照组(维生素E,50 mg·kg-1),连续给药8周后检测血及脑组织中的超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)和总抗氧化能力(T-AOC)等指标。结果:与模型对照组比较,小叶丁香总皂苷低剂量组能明显升高血清中的SOD、GSH-Px和CAT活性以及脑组织中的T-AOC活性;小叶丁香高剂量组能显著降低小鼠血清及脑组织中的MDA水平,升高小鼠血清中的SOD、GSH-Px、CAT和T-AOC活性以及脑组织中的SOD、GSH-Px和T-AOC活性。结论:小叶丁香总皂苷能抑制机体内脂质过氧化物的生成,增强抗氧化酶的活性,从而提高衰老小鼠的抗氧化能力,延缓衰老。
[Abstract]:Objective: to investigate the anti-oxidation effect of total saponins of clove on aging model mice induced by D-galactose. Methods: the aging model was established by subcutaneous injection of D-galactose (200mg kg-1 d-1) into the neck and back of mice. The superoxide dismutase (SOD), malondialdehyde (malondialdehyde) and glutathione (glutathione) in blood and brain tissues were detected after 8 weeks of administration of clove lobule total saponins (60 mg kg-1), high dosage group (120mg kg-1) and positive drug control group (50 mg kg-1). Peptide peroxidase (GSH-Px), catalase (cat) and total antioxidant capacity (T-AOC). Results: compared with the model control group, the low dose group could significantly increase the activities of GSH-Px and cat in serum and T-AOC activity in brain tissue, and the MDA level in serum and brain tissue was significantly decreased in the high dose group of clove lobule. The activities of GSH-Px cat and T-AOC in serum and the activities of GSH-Px and T-AOC in brain tissue were increased. Conclusion: the total saponins of clove can inhibit the production of lipid peroxides, enhance the activity of antioxidant enzymes, improve the antioxidant ability of aging mice and delay senescence.
【作者单位】：黄石市中心医院;
【分类号】：R285.5;R-332

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