外源褪黑素对LPS致宫内感染胎鼠脑组织氧化损伤及TNF-α表达的影响

发布时间：2018-07-18 16:09

【摘要】：目的: 建立大鼠宫内感染模型,研究外源褪黑素(melatonin,MT)对LPS致宫内感染胎鼠脑组织氧化损伤及TNF-α的表达变化,并探讨MT对宫内感染胎鼠脑组织的保护作用。方法: 选用妊娠第19天SD大鼠随机分组,对照组(S组)、宫内感染组(LPS组)和MT处理组(MT组)。通过给孕鼠腹腔注射细菌脂多糖(1ipopolysaccharide,LPS)造成大鼠宫内感染模型。LPS组和S组分别予腹腔注射LPS(500μg/kg)和等容积生理盐水;MT处理组孕鼠同时腹腔注射LPS 500μg/kg +MT 10mg/kg。根据注药后观察时间不同,将各组孕鼠分为注药后1h、6h、12h、24h、48h、72h共6个时间点,每个时间点4只孕鼠,于相应的时间点迅速处死孕鼠,冰盒上剖腹取胎并随机取出8只胎鼠脑组织,分别测定脑组织匀浆的MDA活力和GSH-Px含量,采用RT-PCR技术检测胎鼠脑组织中TNF-αmRNA的水平表达情况,并比较各组之间的差异。结果: 1、成功构建了宫内感染胎鼠脑损伤模型。 2、随感染时间延长,宫内感染组较对照组胎鼠脑组织中MDA活力上升,GSH-Px含量下降,TNF-αmRNA含量则是先升后降;同时随着感染时间的延长,上述改变趋于明显(P0.05)。 3、外源MT能使宫内感染胎鼠脑组织中MDA活力显著降低,明显升高GSH-Px含量,TNF-αmRNA水平显著下降,且均有统计学意义(P 0.05)。结论: 1、通过给孕鼠腹腔注射脂多糖成功建立了宫内感染模型并可致胎鼠脑损伤,表明宫内感染是导致胎鼠脑损伤的重要因素之一。 2、宫内感染致胎鼠脑组织存在氧化损伤、脑神经损伤增多,同时一定时间内,随着感染时间的延长而逐渐加重。 3、褪黑素对宫内感染胎鼠脑组织有抗氧化、抗损伤的作用,并能抑制和降低宫内感染诱导的炎症因子的增加,表明褪黑素对宫内感染胎鼠脑组织有保护作用。
[Abstract]:Aim: to establish a rat model of intrauterine infection and to study the effects of exogenous melatonin (MT) on oxidative damage and expression of TNF- 伪 in fetal brain tissue induced by lipopolysaccharide (LPS), and to explore the protective effect of MT on fetal brain tissue infected with intrauterine infection. Methods: SD rats on the 19th day of pregnancy were randomly divided into control group (S group), intrauterine infection group (LPS group) and MT treatment group (MT group). Intraperitoneal injection of lipopolysaccharide (LPS) by intraperitoneal injection of lipopolysaccharide (LPS) in pregnant rats. The intrauterine infection model of rats was induced by intraperitoneal injection of lipopolysaccharide (LPS). Group S and group S were treated by intraperitoneal injection of LPS (500 渭 g/kg) and intraperitoneal injection of LPS 500 渭 g/kg MT (10 mg / kg) at the same time. According to the different observation time, the pregnant rats in each group were divided into 6 time points at 1h, 6h, 12h, 24h, 48h and 72h. At each time point, 4 pregnant rats were killed quickly at the corresponding time points. The fetal tissues were taken out by caesarean section on the ice box and 8 fetal brain tissues were taken out at random. The activity of MDA and the content of GSH-Px in brain homogenate were measured, and the expression of TNF- 伪 mRNA in fetal brain tissue was detected by RT-PCR. Results: 1. The rat model of fetal brain injury caused by intrauterine infection was successfully constructed. (2) with the prolongation of the time of infection, the content of GSH-Px in the intrauterine infection group was lower than that in the control group, and the content of TNF- 伪 mRNA increased first and then decreased; At the same time, with the extension of infection time, the above changes tended to be obvious (P0.05). Exogenous MT could significantly decrease the activity of MDA and increase the content of GSH-Px and decrease the level of TNF- 伪 mRNA significantly (P 0.05). Conclusion: 1. Intrauterine infection model was successfully established by intraperitoneal injection of lipopolysaccharide into pregnant rats, and fetal brain injury was induced. The results indicate that intrauterine infection is one of the important factors leading to fetal brain injury. With the prolongation of infection time, melatonin can inhibit and decrease the increase of inflammatory factors induced by intrauterine infection. These results suggest that melatonin has protective effect on fetal brain tissue infected by intrauterine infection.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363

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