高脂环境下雄激素缺乏对小型猪内脏脂肪蓄积和炎症基因表达的影响

发布时间：2018-07-20 19:04

【摘要】：目的探讨高脂环境下雄激素缺乏对小型猪内脏脂肪沉积、血清激素以及炎症相关基因表达的影响。方法将性成熟的雄性五指山小型猪随机分成三组,即不去势组(SHAM),去势组(CAS)和去势加睾酮组(CAS+T),每组6只,所有动物均饲喂高脂饲料。12周实验结束时,采血检测血清激素水平变化,分离内脏脂肪并进行称重,采用荧光定量PCR技术检测脂肪合成与脂肪分解基因以及炎症相关基因的表达。结果 (1)去势显著减少高脂饮食小型猪血清睾酮含量但增加其血清瘦素含量,给予外源性睾酮处理能够恢复去势小型猪血清睾酮和瘦素水平;(2)去势导致高脂饮食小型猪内脏脂肪大量沉积,给予睾酮处理后,去势小型猪内脏脂肪含量显著降低;(3)去势和睾酮处理对高脂饮食小型猪内脏脂肪组织FAS、ACC、HSL和ATGL等脂肪代谢基因的表达没有显著影响;(4)去势显著上调高脂饮食小型猪内脏脂肪组织Leptin、CD68、CCL16、CCL23和SAA等炎症基因的表达,采用外源性睾酮处理去势小型猪则能降低上述基因的表达。结论去势诱导的雄激素缺乏促进高脂饮食小型猪内脏脂肪沉积,并上调其内脏脂肪组织中炎症基因的表达,采用睾酮处理则能够改善去势小型猪内脏脂肪蓄积和炎症反应。
[Abstract]:Objective to investigate the effect of androgen deficiency on visceral adipose deposition, serum hormone and expression of inflammation related genes in small pigs. Methods the sexually mature male Five Fingers Group miniature pigs were randomly divided into three groups, namely, the non castration group (SHAM), the castration group (CAS) and the castrated and testosterone group (CAS+T), with 6 rats in each group. All the animals were fed with high fat diet. At the end of the 12 week experiment, serum hormone levels were detected, visceral fat was separated and weighed. The expression of fat synthesis and fat decomposition gene and inflammation related genes were detected by fluorescence quantitative PCR. Results (1) the castration significantly reduced the content of serum albumin in the blood of small pigs with high fat diet but increased the content of serum leptin. Testosterone treatment could restore serum testosterone and leptin levels in castrated miniature pigs; (2) castration resulted in a large amount of visceral fat deposition in high fat diet miniature pigs, and the visceral fat content of castrated miniature pigs decreased significantly after testosterone treatment, and (3) castration and testosterone treatment on fatty tissue such as FAS, ACC, HSL and ATGL in high fat drinking miniature pigs The expression of the Xie gene was not significantly affected; (4) the expression of Leptin, CD68, CCL16, CCL23 and SAA in the visceral adipose tissues of high fat diet small pigs was significantly up-regulated, and the expression of the above gene could be reduced by exogenous testosterone treatment of castrated miniature pigs. Conclusion the androgen deficiency induced by the castration induced visceral fat in high fat diet miniature pigs Lipid deposition and upregulating the expression of inflammatory genes in visceral adipose tissue can improve visceral fat accumulation and inflammatory response in ovariectomized miniature pigs by testosterone treatment.
【作者单位】：浙江中医药大学动物实验研究中心/比较医学研究中心;
【基金】：浙江省公益性技术应用研究(实验动物)计划项目(编号:2014C37010) 国家自然科学基金项目(编号:31200921)
【分类号】：R589.2;R-332

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