红树林淡紫拟青霉胞外多糖对小鼠DCs吞噬功能的影响
发布时间：2018-07-21 17:05
【摘要】：目的探讨红树林来源的淡紫拟青霉胞外多糖对小鼠骨髓源性树突细胞(DCs)功能成熟的影响。方法从小鼠骨髓腔中分离获得骨髓细胞,加入重组小鼠粒细胞-巨噬细胞集落刺激因子(rmGM-CSF)、重组小鼠白细胞介素-4(rmIL-4)诱导分化为未成熟DCs,用不同浓度淡紫拟青霉胞外多糖干预,流式细胞术检测DCs的表面标志CD11c、主要组合相容性复合体(MHCⅡ)类分子、CD80、CD86的表达情况及吞噬葡聚糖(FITC-dextran)的能力,反转录-聚合酶链反应(RT-PCR)检测该多糖对DCs Toll样受体(TLR)2mRNA和TLR4mRNA表达的影响。结果经300、400μg/mL多糖作用48h后DCs表面分子CD11c、MHCⅡ类分子、CD80、CD86的表达较空白对照组显著上调(P0.01);经多糖作用的DCs吞噬FITC-dextran能力下降,尤其是300、400μg/mL的多糖与空白对照组相比作用效果明显(P0.05);另外,该多糖还可下调DCs TLR2mRNA和TLR4mRNA的表达,尤其经100~400μg/mL多糖处理的DCs下调作用显著,与空白对照组相比差异有统计学意义(P0.05)。结论淡紫拟青霉胞外多糖可上调小鼠骨髓源性未成熟DCs表面CD11c、MHCⅡ类分子、CD80和CD86的表达,降低其吞噬能力,下调TLR2mRNA和TLR4mRNA的表达,初步表明该多糖可刺激DCs分化成熟。
[Abstract]:Objective to investigate the effect of mangrove derived Penicillium lilicum extracellular polysaccharide on the functional maturation of mouse bone marrow derived dendritic cells (DCs). Methods bone marrow cells were isolated from the bone marrow cavity of mice, and the recombinant mouse granulocyte macrophage colony stimulating factor (rmGM-CSF) was added to the mice. The induced differentiation of interleukin -4 (rmIL-4) in the mice was induced into immature mice. DCs, using different concentrations of Paecilomyces lilici extracellular polysaccharide intervention, flow cytometry detection of the surface markers of DCs CD11c, the main combinatorial complex (MHC II) molecules, CD80, CD86 expression and the ability to phagocytic dextran (FITC-dextran), reverse transcription polymerase chain reaction (RT-PCR) detection of the polysaccharide on DCs Toll like receptor (TLR) 2mRNA and exports The effect of the expression of 4mRNA. Results the expression of DCs surface molecules CD11c, MHC II, CD80, CD86 was significantly up (P0.01) after the action of 300400 mu g/mL polysaccharide, CD80, CD86, and the FITC-dextran ability of the DCs was decreased, especially the 300400 micron g/mL polysaccharide was significantly more effective than the empty white control group. Polysaccharides could also reduce the expression of DCs TLR2mRNA and TLR4mRNA, especially the down-regulation of DCs treated with 100~400 mu g/mL polysaccharide, and there was a significant difference between the control group and the blank control group (P0.05). Conclusion PP can increase the CD11c, MHC II, CD80 and CD86. The ability to downregulate TLR2mRNA and TLR4mRNA expression indicated that the polysaccharide could stimulate DCs differentiation and maturation.
【作者单位】： 海南医学院临床学院;海南医学院热带医学与检验学院;
【基金】：国家自然科学基金资助项目(31260225) 海南省自然科学基金资助项目(813248) 海南医学院科研培育基金资助项目(HY2014-015)
【分类号】：R392
本文编号：2136203
[Abstract]:Objective to investigate the effect of mangrove derived Penicillium lilicum extracellular polysaccharide on the functional maturation of mouse bone marrow derived dendritic cells (DCs). Methods bone marrow cells were isolated from the bone marrow cavity of mice, and the recombinant mouse granulocyte macrophage colony stimulating factor (rmGM-CSF) was added to the mice. The induced differentiation of interleukin -4 (rmIL-4) in the mice was induced into immature mice. DCs, using different concentrations of Paecilomyces lilici extracellular polysaccharide intervention, flow cytometry detection of the surface markers of DCs CD11c, the main combinatorial complex (MHC II) molecules, CD80, CD86 expression and the ability to phagocytic dextran (FITC-dextran), reverse transcription polymerase chain reaction (RT-PCR) detection of the polysaccharide on DCs Toll like receptor (TLR) 2mRNA and exports The effect of the expression of 4mRNA. Results the expression of DCs surface molecules CD11c, MHC II, CD80, CD86 was significantly up (P0.01) after the action of 300400 mu g/mL polysaccharide, CD80, CD86, and the FITC-dextran ability of the DCs was decreased, especially the 300400 micron g/mL polysaccharide was significantly more effective than the empty white control group. Polysaccharides could also reduce the expression of DCs TLR2mRNA and TLR4mRNA, especially the down-regulation of DCs treated with 100~400 mu g/mL polysaccharide, and there was a significant difference between the control group and the blank control group (P0.05). Conclusion PP can increase the CD11c, MHC II, CD80 and CD86. The ability to downregulate TLR2mRNA and TLR4mRNA expression indicated that the polysaccharide could stimulate DCs differentiation and maturation.
【作者单位】： 海南医学院临床学院;海南医学院热带医学与检验学院;
【基金】：国家自然科学基金资助项目(31260225) 海南省自然科学基金资助项目(813248) 海南医学院科研培育基金资助项目(HY2014-015)
【分类号】：R392
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