糖鞘脂分析技术及相关疾病研究进展
发布时间：2018-07-22 21:20
【摘要】：糖鞘脂是一类广泛分布在动物细胞膜表面的糖脂类物质,它在调控细胞识别、黏附、增殖以及凋亡等方面均有重要的生物学作用.本综述主要讨论了在现代分析技术范畴中,糖鞘脂的鉴定及其糖链结构的分离与解析方面的研究进展和糖鞘脂在癌症等疾病发生发展中所起的生物学功能,以及糖鞘脂作为疾病治疗靶标的可能性.随着现代仪器技术,尤其是质谱技术和色谱-质谱联用技术的发展,糖鞘脂的分离与检测也进入了高速发展的时代.目前,使用质谱技术在肝癌、结直肠癌、乳腺癌等恶性肿瘤的组织样本中均发现了不同种类糖鞘脂不同程度的异常表达.其中,岩藻糖基化的糖鞘脂上调表达在众多癌症糖鞘脂检测中尤为突出,故岩藻糖基化的糖鞘脂可能会成为一类癌症的早期诊断标志物.近年来,随着对糖鞘脂理解的不断深入,糖鞘脂在诸多疾病,如癌症血管生成过程中的功能研究成为了热点之一.例如,从肿瘤细胞表面脱落的大多数糖鞘脂在肿瘤微环境中主要起到了促进血管生成的作用,而与此相反的是,另一种结构简单的神经节苷脂GM3却起到了抑制血管生成的作用.本综述汇集了对上述现象在分子水平上的不同解读以及利用此现象对癌症靶向治疗的研究与探索,并对基于抑制糖鞘脂合成的靶向治疗的发展前景进行了分析展望.
[Abstract]:Saccharin is a kind of glycolipids widely distributed on the surface of animal cell membrane. It plays an important biological role in regulating cell recognition, adhesion, proliferation and apoptosis. In this review, the recent advances in the identification of sphingolipids, the separation and analysis of their sugar chain structures, and their biological functions in the occurrence and development of cancer and other diseases are mainly discussed in the field of modern analytical techniques. And the possibility of saccharin as a target for disease treatment. With the development of modern instrument technology, especially mass spectrometry and chromatography-mass spectrometry, the separation and detection of glycolipid has entered the era of rapid development. At present, mass spectrometry has been used to detect abnormal expression of different kinds of sphingolipid in tissues of liver cancer, colorectal cancer, breast cancer and other malignant tumors. Among them, the up-regulation of sphingolipid by fucosylation is especially prominent in many cancers, so the glycosylated sphingolipid may become an early diagnostic marker for a class of cancer. In recent years, with the deepening of understanding of glycolipid, the functional study of glycolipid in many diseases, such as cancer angiogenesis, has become one of the hot spots. For example, most of the sphingolipids removed from the surface of tumor cells play a major role in promoting angiogenesis in the tumor microenvironment, whereas another simple ganglioside GM3 inhibits angiogenesis. This review brings together different interpretations of the above phenomena at molecular level, as well as the research and exploration of cancer targeted therapy using this phenomenon, and the prospect of targeted therapy based on inhibition of glycolipid biosynthesis is analyzed and prospected.
【作者单位】： 西北大学生命科学学院功能糖组学实验室;
【基金】：国家自然科学基金基地建设能力提高项目(J1210063)资助~~
【分类号】：Q26;R363
本文编号：2138609
[Abstract]:Saccharin is a kind of glycolipids widely distributed on the surface of animal cell membrane. It plays an important biological role in regulating cell recognition, adhesion, proliferation and apoptosis. In this review, the recent advances in the identification of sphingolipids, the separation and analysis of their sugar chain structures, and their biological functions in the occurrence and development of cancer and other diseases are mainly discussed in the field of modern analytical techniques. And the possibility of saccharin as a target for disease treatment. With the development of modern instrument technology, especially mass spectrometry and chromatography-mass spectrometry, the separation and detection of glycolipid has entered the era of rapid development. At present, mass spectrometry has been used to detect abnormal expression of different kinds of sphingolipid in tissues of liver cancer, colorectal cancer, breast cancer and other malignant tumors. Among them, the up-regulation of sphingolipid by fucosylation is especially prominent in many cancers, so the glycosylated sphingolipid may become an early diagnostic marker for a class of cancer. In recent years, with the deepening of understanding of glycolipid, the functional study of glycolipid in many diseases, such as cancer angiogenesis, has become one of the hot spots. For example, most of the sphingolipids removed from the surface of tumor cells play a major role in promoting angiogenesis in the tumor microenvironment, whereas another simple ganglioside GM3 inhibits angiogenesis. This review brings together different interpretations of the above phenomena at molecular level, as well as the research and exploration of cancer targeted therapy using this phenomenon, and the prospect of targeted therapy based on inhibition of glycolipid biosynthesis is analyzed and prospected.
【作者单位】： 西北大学生命科学学院功能糖组学实验室;
【基金】：国家自然科学基金基地建设能力提高项目(J1210063)资助~~
【分类号】：Q26;R363
【相似文献】
相关会议论文 前3条
1 台桂花;;糖鞘脂及其配体的囊泡转运[A];第十次中国生物物理学术大会论文摘要集[C];2006年
2 裴波;刘志萍;陈建文;;小浓度神经节甘脂GM1对蛋白激酶C活性的调控[A];中国生物化学与分子生物学会第八届会员代表大会暨全国学术会议论文摘要集[C];2001年
3 赵玉峰;刘朝英才;夏泉;徐跃飞;马克里;;脂筏与非脂筏质膜微域磷脂组份及结构的比较分析[A];2008年全国糖生物学学术会议论文摘要[C];2008年
相关硕士学位论文 前1条
1 宋波;糖鞘脂Gg4及其合成酶β3Ga1T4的表达调控机制及功能研究[D];江南大学;2014年
，本文编号：2138609
本文链接：https://www.wllwen.com/xiyixuelunwen/2138609.html
最近更新
教材专著
