细粒棘球绦虫延伸因子1生物信息学分析

发布时间：2018-07-29 12:01

【摘要】：目的利用生物信息学技术对细粒棘球绦虫的延伸因子1(EF-1)进行分析,探讨其诊断价值。方法通过Expasy系统预测EF-1的理化性质,使用DNAStar软件分析蛋白亲水性、柔性区域、抗原指数及表面可及性,利用ABCpred与IEDB软件综合预测B细胞线性抗原表位,通过DNAStar软件预测T细胞表位,使用SOPMA软件预测二级结构,SWISS-MODEL网站构建EF-1的三级结构,使用MEGA软件选择neighbor joining法构建EF-1氨基酸序列的系统发育树。结果 EF-1基因序列全长1 412 bp,含有2个内含子;EF-1亲水性得分较高的区域为35~45、52~70、126~140、158~183、301~322、357~380、424~448;柔性区域为26~45、54~71、162~176、300~318、357~381、432~448;抗原性指数得分较高的区域分布与柔性区域一致;表面可及性得分较高的区域较少,主要分布在39~54、62~76、128~132、161~170、220~234、315~329、354~372、424~448;可能的B细胞线性表位的氨基酸序列为120~130、286~295、306~320、365~378;可能的优势性T细胞表位区域为34~48、164~177、222~244、280~290、321~330、396~407;EF-1的二级结构中α螺旋占32.81%、β折叠占22.54%、β转角占10.94%、无规则卷曲占33.71%;系统发育树结果表明多房棘球绦虫为一枝,其余聚于一枝,且内部形成许多梳齿状分枝。结论得到4个可能形成B细胞表位的区域、6个优势性T细胞表位区域,EF-1具有较高的保守性,可作为免疫诊断和药物治疗靶点。
[Abstract]:Objective to study the diagnostic value of Echinococcus granulosus extension factor 1 (EF-1) by bioinformatics. Methods the physicochemical properties of EF-1 were predicted by Expasy system. The hydrophilicity, flexibility, antigen index and surface accessibility of protein were analyzed by DNAStar software. The linear antigen epitopes of B cells were predicted by ABCpred and IEDB software. DNAStar software was used to predict T cell epitopes, SOPMA software was used to predict secondary structure and SWISS-MODEL website was used to construct the tertiary structure of EF-1. The phylogenetic tree of EF-1 amino acid sequence was constructed by MEGA software neighbor joining method. Results the total length of EF-1 gene sequence was 1 412 BP, and the high hydrophilicity score of EF-1 containing two introns was 35455 / 5270126 / 1401586 / 143301/ 32235757380424/ 448, and the flexible region was 26455/ 555 / 5471162176300318357357381432 / 448 / 448 respectively. The area with higher score of antigenicity index was the same as that of flexible region, and the area with higher surface accessibility score was less. The main amino acid sequence of the possible linear epitope of B cell is 120,130286295306,3203130286295306,320365378; the potential dominant T cell epitope region is 3444816164177222222228240290321330396F-1 in which 伪 helix is 32.811e, 尾 folding is 22.54g, 尾 rotation is 10.9410.910.910.910.71H; the phylogenetic tree has a secondary structure of 32.811e, 尾 folding is 22.54g, 尾 turn is 10.9410.910.910.910.910.910.91.The phylogenetic tree has a secondary structure of "伪 helix" (32.811g), 尾 folding (22.54g), 尾 turning angle (10.9410.94) and irregular curling (33.71a); and phylogenetic tree development tree (phylogenetic tree). The results showed that Echinococcus multilocularis was one branch. The rest gather in one branch and form many comb-like branches inside. Conclusion there are 4 B cell epitopes and 6 dominant T cell epitopes, which are highly conserved and can be used as targets for immunological diagnosis and drug therapy.
【作者单位】：青海大学医学院病原生物学教研室;青海大学包虫病重点实验室;
【基金】：国家自然科学基金(81360255)
【分类号】：R383.33

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