丘脑腹内侧核高频电刺激改善帕金森病模型大鼠前肢使用不对称行为的研究

发布时间：2018-07-29 14:22

【摘要】：目的探讨丘脑腹内侧核高频电刺激能否改善帕金森病模型大鼠前肢使用不对称行为。方法将33只SD大鼠随机分为实验组22只和对照组11只。利用立体定向的方法将刺激电极埋入右侧丘脑腹内侧核,用于注射6-羟基多巴胺的套管埋入右侧内侧前脑束。实验组大鼠右侧内侧前脑束内注射12μg的6-OHDA,对照组注射相同体积的生理盐水。对造模前、造模后和给予DBS时的大鼠进行前肢使用不对称测试。黑质酪氨酸羟化酶免疫组化染色检测黑质多巴胺能神经元毁损程度。尼氏染色确定刺激电极尖端位置。结果从22只大鼠中获得了15只成功的PD大鼠模型。与毁损前相比,模型大鼠毁损后的不对称指数显著增大(P=0.000)。给予高频VM-DBS后,不对称指数显著下降,与毁损后未给予VM-DBS时比较,差异有统计学意义(P=0.009);而与毁损前相比,差异不显著(P=0.214)。给予低频VM-DBS后,不对称指数无明显改变,与毁损后未给予VM-DBS时比较,差异无统计学意义(P=0.4);而与毁损前相比,差异显著(P=0.036)。结论丘脑腹内侧核高频电刺激能够改善6-羟基多巴胺偏侧帕金森病模型大鼠的前肢使用不对称行为,丘脑腹内侧核可能是帕金森病深部脑刺激治疗的潜在有效靶核团。
[Abstract]:Objective to investigate whether high frequency stimulation of ventromedial nucleus of thalamus can improve the asymmetric behavior of forelimb in Parkinson's disease model rats. Methods 33 SD rats were randomly divided into experimental group (n = 22) and control group (n = 11). The stimulation electrode was implanted into the ventromedial nucleus of the right thalamus by stereotactic method and the 6-hydroxydopamine cannula was injected into the right medial forebrain tract. The right medial forebrain of the experimental group was injected with 12 渭 g of 6-OHDAand the control group was injected with the same volume of normal saline. The antebrachial asymmetry test was performed on rats before, after and after DBS administration. The damage of substantia nigra dopaminergic neurons was detected by tyrosine hydroxylase immunohistochemical staining. The tip position of the stimulation electrode was determined by Nissl staining. Results Fifteen successful PD rat models were obtained from 22 rats. The asymmetry index of model rats increased significantly (P0. 000). After high frequency VM-DBS, the asymmetry index decreased significantly (P 0. 009), but there was no significant difference (P 0. 214). There was no significant change in asymmetry index after low frequency VM-DBS, but there was no significant difference (P0. 036) between the two groups when VM-DBS was not given after the damage (P0. 4), but significant difference was found between the two groups (P0. 036). Conclusion High frequency electrical stimulation of ventromedial nucleus of thalamus can improve the asymmetric behavior of the forelimb of 6-hydroxydopamine hemiparkinsonism rats. The ventromedial nucleus of thalamus may be a potentially effective target nucleus for deep brain stimulation in Parkinson's disease.
【作者单位】：海南医学院附属医院神经外科;南方医科大学珠江医院神经外科;青海大学附属医院神经外科;中国科学院心理研究所;陆军总医院附属八一脑科医院;
【基金】：国家自然科学基金面上项目(编号:30872662) 海南医学院科研培育基金项目(编号:HY2015-19)
【分类号】：R742.5;R-332

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