黑猩猩和人类左脑成纤维细胞生长因子1（FGF1）不同分子网络和机制的功能预测
发布时间：2018-07-31 19:24
【摘要】：在本文中,我们的目标是分别建立黑猩猩左脑和人类左脑的上下游激活和抑制网络。我们使用的芯片中,包含了12558个来自于15个黑猩猩左脑样本和14个人类左脑样本的基因。我们所使用的显著性表达基因标记,是通过使用微阵列的显著性差异分析方法而得到的。我们通过将数据进行log2的归一化处理,并运用聚类分析方法进行分析。首先,我们使用GRNInfer工具,构建了441个具有显著性高和低表达分子的整体网络；第二,我们从已构建的黑猩猩和人类左脑的整体网络中,确定了成纤维细胞生长因子1 (FGF1)的表达特性；第三,我们确定了黑猩猩和人类左脑的成纤维细胞生长因子1 (FGF1)的上下游网络；第四,我们从黑猩猩和人类左脑的成纤维细胞生长因子1 (FGF1)的上下游网络中进一步确认了分子的激活和抑制特性。我们使用的生物学分析方法已经得到了生物医学领域相关专家的承认,并给予了高度评价,所发表的论文分别刊登在SCI检索的国际杂志上,例如Cell Biochemistry and Biophysics (影响因子4.311), Journal of Cellular Biochemistry(影响因子3.121), Cell Proliferation (影响因子2.742),Cellular and Molecular Neurobiology (影响因子2.423)。 在本文中,我们通过GO数据库中的基因数据分析发现,分子FGF1的相关模块包括：细胞外区,细胞外基质,细胞外空间,蛋白结合,生长因子活性,肝素结合,血管生成,器官诱导,信号传导,发育,细胞增殖,成细胞生长因子受体信号通路,形态发生,细胞分化,肺发育,上皮细胞增殖的正调控。我们通过将黑猩猩的左脑和人类左脑进行比较,从而在黑猩猩的左脑中分别构建了低表达的成纤维细胞生长因子1 (FGF1)的DNA损伤介导的双链断裂重组修复,耦合了细胞分裂到端粒维持的抗凋亡网络和成纤维细胞生长因子1 (FGF1)的先天免疫驱动的转录耦合端粒维持的缩短导致细胞凋亡的调控网络。而成纤维细胞生长因子1 (FGF1)在人类左脑中,当基因表达改变最小倍数设为2倍时表现为高表达的特性。我们在人类左脑中分别构建了高表达的成纤维细胞生长因子1 (FGF1)的防御响应介导的迁移耦合绑定运输的信号到氧化代谢的诱导树突发育网络和高表达的成纤维细胞生长因子1 (FGF1)的DNA修复的迁移耦合到轴突扩建正调控转录抑制网络。我们的研究结果表明,在黑猩猩左脑中,上游激活网络：分子C10ORF10, CDC25B, LOH11CR2A, RAD50, SAPS2, STAMBP激活FGF1;而在下游的激活网络中,FGF1没有激活任何分子；上游抑制网络：AL049278, AL080232, CFHR1, CTBP1, DDX3Y, RNF2, RPP14, SARM1, TERF1_1抑制FGF1;而下游抑制网络没有相应的结果。在人类的左脑中,上游激活网络：分子CTRL, GPD1, LGALS3BP, MAP1B_3, PCDHGA8, PCSK6, PDIA2激活FGF1;而在下游的激活网络中,FGF1激活分子MGC15523, NUPR1, UBXD2;上游网络：DTNA, FOXN3_1, MAPT, NAIP, NR1D2_2, SLC25A46, SMG1抑制FGF1;而下游网络：FGF1抑制分子ISCA1, PSMA4, U79289。我们对成纤维细胞生长因子1 (FGF1)的研究对于神经退化性疾病的研究以及临床治疗具有重要作用,对于利用基因进行疾病的病理性研究具有重要意义。
[Abstract]:In this article, our goal is to establish the upstream and downstream activation and suppression networks of the left brain of the chimpanzee and the human left brain. In the chips we use, we include 12558 genes from 15 chimpanzee left brain samples and 14 human left brain samples. The method of sex difference analysis is obtained. We analyze the data by normalizing the log2 and using clustering analysis. First, we use the GRNInfer tool to build 441 overall networks with significant and low expression molecules; second, we are from the built chimpanzee and the human left brain as a whole network, The expression characteristics of fibroblast growth factor 1 (FGF1) were determined; third, we identified the upstream and downstream networks of chimpanzee and human left brain fibroblast growth factor 1 (FGF1); fourth, we further confirmed the activation of the molecules from the upstream and downstream networks of the fibroblast growth factor 1 (FGF1) of the left brain of the human and the human left brain. The biological analysis methods we use have been recognized by relevant experts in the field of biomedicine and are highly evaluated. The published papers are published in the International Journal of SCI retrieval, such as Cell Biochemistry and Biophysics (influence factor 4.311), Journal of Cellular Biochemistry (influence factor 3) .121), Cell Proliferation (influence factor 2.742), Cellular and Molecular Neurobiology (influence factor 2.423).
In this paper, we found that the related modules of molecular FGF1 include extracellular domain, extracellular matrix, extracellular space, protein binding, growth factor activity, heparin binding, angiogenesis, organ induction, signal transduction, propagation, cell proliferation, cell growth factor receptor signaling pathway, morphologic development. The positive regulation of cell differentiation, lung development and epithelial cell proliferation. We have compared the left brain of chimpanzee with the human left brain, thus constructing a low expressed DNA damage mediated double strand break recombination repair in the left brain of the chimpanzee, which coupled cell division to telomere maintenance. Apoptotic network and fibroblast growth factor 1 (FGF1), the shortening of the congenital immune driven transcription coupling telomere maintenance, which leads to apoptosis, and fibroblast growth factor 1 (FGF1) in the human left brain, when the minimum multiplier of gene expression is set to 2 times as high expression. We divide the human left brain into the human left brain. Do not construct a highly expressed fibroblast growth factor 1 (FGF1) defense response mediated transport coupled binding transport signal to oxidative metabolism induced dendritic development network and high expression of fibroblast growth factor 1 (FGF1) DNA repair transfer coupling to the axon expansion positive regulatory transcriptional inhibition network. In the left brain of chimpanzee, the upstream activation network: molecules C10ORF10, CDC25B, LOH11CR2A, RAD50, SAPS2, STAMBP activate FGF1; and in the downstream activation network, FGF1 does not activate any molecules; the upstream suppression network: AL049278, AL080232, CFHR1, CTBP1, CTBP1, repressive, restraining networks; and downstream inhibition networks In the human left brain, the upstream activation network: molecular CTRL, GPD1, LGALS3BP, MAP1B_3, PCDHGA8, PCSK6, PDIA2 activate FGF1; and in the downstream activation network, FGF1 activator MGC15523, NUPR1, UBXD2; upstream network: downstream network: GF1 inhibitory molecules ISCA1, PSMA4, U79289., our study of fibroblast growth factor 1 (FGF1) has an important role in the research and clinical treatment of neurodegenerative diseases, and is of great significance for the use of gene for pathological study of disease.
【学位授予单位】：北京邮电大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363
本文编号：2156589
[Abstract]:In this article, our goal is to establish the upstream and downstream activation and suppression networks of the left brain of the chimpanzee and the human left brain. In the chips we use, we include 12558 genes from 15 chimpanzee left brain samples and 14 human left brain samples. The method of sex difference analysis is obtained. We analyze the data by normalizing the log2 and using clustering analysis. First, we use the GRNInfer tool to build 441 overall networks with significant and low expression molecules; second, we are from the built chimpanzee and the human left brain as a whole network, The expression characteristics of fibroblast growth factor 1 (FGF1) were determined; third, we identified the upstream and downstream networks of chimpanzee and human left brain fibroblast growth factor 1 (FGF1); fourth, we further confirmed the activation of the molecules from the upstream and downstream networks of the fibroblast growth factor 1 (FGF1) of the left brain of the human and the human left brain. The biological analysis methods we use have been recognized by relevant experts in the field of biomedicine and are highly evaluated. The published papers are published in the International Journal of SCI retrieval, such as Cell Biochemistry and Biophysics (influence factor 4.311), Journal of Cellular Biochemistry (influence factor 3) .121), Cell Proliferation (influence factor 2.742), Cellular and Molecular Neurobiology (influence factor 2.423).
In this paper, we found that the related modules of molecular FGF1 include extracellular domain, extracellular matrix, extracellular space, protein binding, growth factor activity, heparin binding, angiogenesis, organ induction, signal transduction, propagation, cell proliferation, cell growth factor receptor signaling pathway, morphologic development. The positive regulation of cell differentiation, lung development and epithelial cell proliferation. We have compared the left brain of chimpanzee with the human left brain, thus constructing a low expressed DNA damage mediated double strand break recombination repair in the left brain of the chimpanzee, which coupled cell division to telomere maintenance. Apoptotic network and fibroblast growth factor 1 (FGF1), the shortening of the congenital immune driven transcription coupling telomere maintenance, which leads to apoptosis, and fibroblast growth factor 1 (FGF1) in the human left brain, when the minimum multiplier of gene expression is set to 2 times as high expression. We divide the human left brain into the human left brain. Do not construct a highly expressed fibroblast growth factor 1 (FGF1) defense response mediated transport coupled binding transport signal to oxidative metabolism induced dendritic development network and high expression of fibroblast growth factor 1 (FGF1) DNA repair transfer coupling to the axon expansion positive regulatory transcriptional inhibition network. In the left brain of chimpanzee, the upstream activation network: molecules C10ORF10, CDC25B, LOH11CR2A, RAD50, SAPS2, STAMBP activate FGF1; and in the downstream activation network, FGF1 does not activate any molecules; the upstream suppression network: AL049278, AL080232, CFHR1, CTBP1, CTBP1, repressive, restraining networks; and downstream inhibition networks In the human left brain, the upstream activation network: molecular CTRL, GPD1, LGALS3BP, MAP1B_3, PCDHGA8, PCSK6, PDIA2 activate FGF1; and in the downstream activation network, FGF1 activator MGC15523, NUPR1, UBXD2; upstream network: downstream network: GF1 inhibitory molecules ISCA1, PSMA4, U79289., our study of fibroblast growth factor 1 (FGF1) has an important role in the research and clinical treatment of neurodegenerative diseases, and is of great significance for the use of gene for pathological study of disease.
【学位授予单位】：北京邮电大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363
【参考文献】
相关期刊论文 前1条
1 王雅丹;胡豫;张璐;孙春艳;;脑源性神经营养因子通过AKT和ERK1/2信号通路诱导内皮细胞血管生成(英文)[J];中国实验血液学杂志;2008年01期
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