健脾益气摄血方对辛伐他汀诱导斑马鱼出血模型的止血效果研究
发布时间：2018-08-01 17:36
【摘要】：目的:观察健脾益气摄血方对辛伐他汀诱导斑马鱼出血的止血效果并与清热凉血方比较。方法:用辛伐他汀0.5μmol/L处理AB系斑马鱼24 h,建立斑马鱼出血模型,在严格盲态实验条件下,在最大非致死量范围内,应用不同浓度实验药物处理出血斑马鱼,以出血减少率、止血率为主要观察指标,以类似脾气虚特点的血流量、血流量改善率与运动速度、运动改善率为附加指标,综合评价健脾益气摄血方的干预效果。结果:实验药物B1在浓度为500和1 000μg/ml时出血斑马鱼的出血率分别为30%和15%,止血率分别为60%和80%;实验药物B2在浓度为500和1 000μg/ml时出血斑马鱼出血率分别为45%和40%,止血率分别为40%和47%。表明在降低斑马鱼出血率与止血效果两方面实验药物B1优于实验药物B2。在相等浓度下,在提高出血斑马鱼血流量与血流量改善率两方面实验药物B1优于实验药物B2。在相等浓度下,在促进斑马鱼运动速度与提高斑马鱼运动改善率方面实验药物B1优于实验药物B2。结论:健脾益气摄血方对辛伐他汀诱导的斑马鱼出血有良好的止血效果,并能够改善出血斑马鱼血流量与提高运动速度,为健脾益气摄血方治疗脾不统血证提供了实验依据。
[Abstract]:Aim: to observe the hemostatic effect of Jianpi Yiqi decoction on zebrafish bleeding induced by simvastatin and compare it with Qingre Liangxue recipe. Methods: AB zebrafish were treated with simvastatin 0.5 渭 mol/L for 24 h. Under strict blind experimental conditions, different concentrations of experimental drugs were used to treat the bleeding zebrafish in the range of maximum non-lethal dose, in order to reduce the bleeding rate. The hemostatic rate was regarded as the main observation index, with the blood flow, the improvement rate of blood flow, the speed of movement and the rate of improvement of exercise as the additional indexes, the intervention effect of the prescription for invigorating spleen and invigorating qi was evaluated synthetically. Results: the bleeding rate of zebrafish was 30% and 15.5%, the hemostatic rate was 60% and 80%, respectively, when the concentration of B1 was 500 渭 g/ml and 1 000 渭 g/ml, the bleeding rate of zebrafish was 45% and 40% when the concentration of drug B2 was 500 渭 g/ml and 1 000 渭 g/ml, respectively. The blood rates were 40% and 47%, respectively. The results showed that the experimental drug B1 was superior to the experimental drug B2 in reducing the bleeding rate and hemostatic effect of zebrafish. At the same concentration, the experimental drug B1 was superior to the experimental drug B2 in increasing the blood flow and improving the blood flow rate of zebrafish. At the same concentration, the experimental drug B1 was superior to the experimental drug B2 in promoting the speed of zebrafish movement and improving the rate of zebrafish movement improvement. Conclusion: Jianpi Yiqi decoction has good hemostatic effect on zebrafish bleeding induced by simvastatin and can improve the blood flow and speed of movement of zebrafish.
【作者单位】： 北京中医药大学东直门医院;杭州环特生物科技股份有限公司;
【基金】：国家重点基础研究发展计划(973计划)课题(2013CB531705)
【分类号】：R285.5;R-332
[Abstract]:Aim: to observe the hemostatic effect of Jianpi Yiqi decoction on zebrafish bleeding induced by simvastatin and compare it with Qingre Liangxue recipe. Methods: AB zebrafish were treated with simvastatin 0.5 渭 mol/L for 24 h. Under strict blind experimental conditions, different concentrations of experimental drugs were used to treat the bleeding zebrafish in the range of maximum non-lethal dose, in order to reduce the bleeding rate. The hemostatic rate was regarded as the main observation index, with the blood flow, the improvement rate of blood flow, the speed of movement and the rate of improvement of exercise as the additional indexes, the intervention effect of the prescription for invigorating spleen and invigorating qi was evaluated synthetically. Results: the bleeding rate of zebrafish was 30% and 15.5%, the hemostatic rate was 60% and 80%, respectively, when the concentration of B1 was 500 渭 g/ml and 1 000 渭 g/ml, the bleeding rate of zebrafish was 45% and 40% when the concentration of drug B2 was 500 渭 g/ml and 1 000 渭 g/ml, respectively. The blood rates were 40% and 47%, respectively. The results showed that the experimental drug B1 was superior to the experimental drug B2 in reducing the bleeding rate and hemostatic effect of zebrafish. At the same concentration, the experimental drug B1 was superior to the experimental drug B2 in increasing the blood flow and improving the blood flow rate of zebrafish. At the same concentration, the experimental drug B1 was superior to the experimental drug B2 in promoting the speed of zebrafish movement and improving the rate of zebrafish movement improvement. Conclusion: Jianpi Yiqi decoction has good hemostatic effect on zebrafish bleeding induced by simvastatin and can improve the blood flow and speed of movement of zebrafish.
【作者单位】： 北京中医药大学东直门医院;杭州环特生物科技股份有限公司;
【基金】：国家重点基础研究发展计划(973计划)课题(2013CB531705)
【分类号】：R285.5;R-332
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