胃得安片对应激性胃损伤模型大鼠胃功能及胃组织中EGF、EGFR表达的影响

发布时间：2018-08-04 10:33

【摘要】：目的:研究胃得安片对应激性胃损伤模型大鼠胃功能及胃组织中表皮生长因子(EGF)及其受体(EGFR)表达的影响,探讨其治疗胃溃疡的作用机制。方法:将大鼠随机分为正常组(生理盐水)、模型组(生理盐水)、阳性组(盐酸雷尼替丁,0.015 mg/kg)和胃得安片高、中、低剂量组(1.7、0.87、0.43 g/kg),每组10只。除正常组外,其余各组大鼠均采用空腹冷水游泳法复制胃溃疡模型。成模后,各组大鼠每天ig给药1次,连续2周。记录大鼠于给药第0、7、14天时的体质量;末次给药12 h后,检测大鼠的胃液分泌量、胃液pH值、胃溃疡面积及胃黏膜损伤指数,并检测胃组织中EGF、EGFR的表达。结果:与正常组比较,模型组大鼠给药第7、14天时的体质量及胃液pH值降低,胃液分泌量和胃溃疡面积增加,胃黏膜损伤指数升高,胃组织中EGF、EGFR表达增强,差异均有统计学意义(P0.05或P0.01)。与模型组比较,除胃得安片中剂量组大鼠给药第7天时的体质量、胃液分泌量、胃液pH值、胃溃疡面积以及胃得安片低剂量组大鼠给药第7、14天时的体质量、胃液分泌量、胃液pH值、胃溃疡面积改善和胃组织中EGF表达增强不显著外,其余各给药组大鼠上述指标均显著改善,且胃组织中EGF、EGFR表达持续增强(P0.05或P0.01);胃得安片的作用呈现一定的量效关系。结论:胃得安片能够显著改善应激性胃损伤模型大鼠的胃功能;其机制可能与增强胃组织中EGF、EGFR表达,从而促进溃疡愈合有关。
[Abstract]:Aim: to study the effects of Weidian tablets on gastric function and the expression of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in gastric tissues of stress induced gastric injury rats, and to explore the mechanism of its action in the treatment of gastric ulcer. Methods: the rats were randomly divided into normal group (normal saline), model group (normal saline), positive group (Ranitidine hydrochloride 0.015 mg/kg) and high, middle and low dose group (1.70.87 0.43 g/kg) with 10 rats in each group. Gastric ulcer was induced by cold water swimming in all groups except the normal group. Rats in each group were given intravenously once a day for 2 weeks. The body weight of the rats was recorded at 714 days after the last administration, and the gastric juice secretion, gastric pH value, gastric ulcer area and gastric mucosal injury index were measured 12 hours after the last administration, and the expression of EGF- EGFR in gastric tissue was detected. Results: compared with the normal group, the body weight and pH value of gastric juice decreased, the gastric juice secretion and gastric ulcer area increased, the gastric mucosal injury index increased, and the EGF- EGFR expression in gastric tissue increased in the model group on the 14th day after administration of the drug. The difference was statistically significant (P0.05 or P0.01). Compared with the model group, the body weight, gastric juice secretion, gastric juice pH value, gastric ulcer area, the body weight and gastric juice secretion of the rats in the middle dose group at 714 days after administration were compared with those in the model group. The pH value of gastric juice, the area of gastric ulcer and the expression of EGF in gastric tissue were not significantly improved, but the above indexes were significantly improved in the other groups (P0.05 or P0.01), and the effect of Wedelan tablets was dose-effect. Conclusion: Weidian tablet can significantly improve gastric function in rats with stress gastric injury, and its mechanism may be related to the enhancement of EGF- EGFR expression in gastric tissue and thus to promote ulcer healing.
【作者单位】：济南军区总医院药剂科;山东中医药大学药学院;
【分类号】：R285.5;R-332

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