腹腔感染大鼠肠粘膜通透性改变与E-cadherin蛋白表达的关系

发布时间：2018-08-04 14:19

【摘要】：目的观察腹腔感染大鼠肠屏障损伤后肠通透性的变化,及其与损伤程度和E-cadherin蛋白表达的相关性。方法健康成年雄性Wistar大鼠40只,随机分为空白对照组(仅行单纯剖腹手术)和腹腔感染(采用盲肠结扎穿孔法制作腹腔感染模型)术后12h、24、36、48h组,每组8只,处死后,距回盲部10cm处取回肠2cm进行病理检查(包括常规检查、测定肠上皮损伤指数)及测定相应时间点血浆D-乳酸含量,应用Western blot方法测定肠粘膜E-cadherin蛋白的水平。结果①腹腔感染12h后肠屏障开始出现明显损伤性改变：肠粘膜损伤评分增加,血浆D-乳酸含量明显增加(P0.01)；24h损伤达到高峰：肠粘膜损伤评分最大,血浆D-乳酸含量明显增高(P0.05)；随后肠屏障逐渐恢复,但48h组损伤程度仍显著高于对照组(P0.01),血浆D-乳酸含量与损伤指数具有显著相关性(r=0.489,P0.01)。②与对照组比较,实验组E-cadherin蛋白表达量降低(P0.05)：12h组、48h组的E-cadherin蛋白水平分别与24h组和36h组比较均有统计学意义(P＜0.05)：24h组和36h组比较无统计学意义(P0.05)。血浆D-乳酸水平与E-cadherin蛋白含量具有明显相关性(r=-0.605,P0.01)。结论腹腔感染状态下,肠屏障严重受损,肠粘膜通透性的改变与肠屏障损伤程度及E-cadherin蛋白水平均具有显著的相关性。
[Abstract]:Objective to observe the changes of intestinal permeability after intestinal barrier injury in rats with abdominal infection and its correlation with injury degree and expression of E-cadherin protein. Methods Forty healthy adult male Wistar rats were randomly divided into two groups: control group (simple caesarean section only) and celiac infection (abdominal infection model made by cecal ligation and perforation) 12 h after operation, 8 rats in each group were killed. The ileal 2cm from ileocecal 10cm was examined by pathological examination (including routine examination, intestinal epithelium injury index) and plasma D-lactic acid content at the corresponding time point. The E-cadherin protein level in intestinal mucosa was measured by Western blot method. Results (1) after 12 hours of intraperitoneal infection, obvious changes of intestinal barrier began to appear: intestinal mucosal injury score increased, plasma D- lactic acid content increased significantly (P0.01) and 24 h injury reached its peak: intestinal mucosal injury score was the highest. Plasma D- lactic acid content was significantly increased (P0.05), then intestinal barrier gradually recovered, but the injury degree of 48h group was still significantly higher than that of the control group (P0.01). There was a significant correlation between the plasma D- lactic acid content and the injury index (r = 0.489g, P0.01), compared with the control group. The expression of E-cadherin protein in the experimental group was significantly lower than that in the control group (P0.05). The level of E-cadherin protein in the 48h group was significantly higher than that in the 24h group and 36h group (P < 0.05). There was no significant difference between the two groups (P0.05). There was a significant correlation between plasma D-lactic acid level and E-cadherin protein content (r-0. 605, P 0. 01). Conclusion under the condition of abdominal infection, the intestinal barrier was seriously damaged, and the change of intestinal mucosal permeability was significantly correlated with the degree of intestinal barrier injury and the level of E-cadherin protein.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363

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