上海地区献血者IgA缺乏调查及CD36缺失表型的初步研究
发布时间：2018-08-07 06:33
【摘要】：免疫球蛋白A(immunoglobulin A, IgA)缺乏一般指血清中IgA含量少于5mg/dL或缺失,当含量低至0.05mg/dL以下时则称为IgA完全缺乏。在IgA缺乏的个体中会产生抗-IgA,当输注含IgA的血液成分时可增加输血过敏反应的发生风险,严重者可致休克或死亡。IgA缺乏症在不同种族中的发生频率已有广泛研究,且存在差异性。中国健康献血者人群中IgA发生率仍未知,且对IgA缺乏者的输血治疗缺少针对性的输血策略。本研究对来自上海及周边地区汉族健康献血者进行IgA缺乏筛查,以获得其发生频率,并初步分析IgA输血过敏反应发生风险,以保障输血安全。通过建立一种双夹心酶联免疫分析(ELISA)方法初步检测22609份健康献血员血清中IgA含量,IgA缺乏者再用凝胶免疫法确认之,同时对IgA缺乏标本作抗-IgA的检测。经统计,22 609份健康献血者标本中,发现14例IgA缺乏者,比例为0.062%(1：1615)。其中7例确认为IgA含量0.05 mg/dL,由此上海地区献血人群IgA完全缺乏比例为0.031%(1：3230)。在这14例IgA缺乏(IgA5 mg/dL)标本中鉴定出2例含抗-IgA；IgA缺乏且含有抗-IgA者比例为0.009%(1：11305)。上海地区健康献血者中IgA缺乏发生频率介于日本人种与高加索人种之间,但对IgA缺乏者的输血仍存在潜在的风险,需要采取一定措施预防和规避输血过敏反应的发生
[Abstract]:Immunoglobulin A (immunoglobulin A, IgA) deficiency generally refers to the IgA content in the serum is less than or absent from 5mg/dL, and when the content is lower than 0.05mg/dL, it is called IgA deficiency. Anti-IgA is produced in individuals with IgA deficiency, which increases the risk of allergic reactions to blood transfusion when infused with blood components containing IgA, and the frequency of shock or death in severe cases in different ethnic groups has been extensively studied. And there are differences. The incidence of IgA in healthy blood donors in China is still unknown, and there is no specific transfusion strategy for patients with IgA deficiency. In order to obtain the frequency of IgA deficiency in healthy blood donors of Han nationality from Shanghai and its surrounding areas, the risk of allergic reaction of IgA transfusion was preliminarily analyzed in order to ensure the safety of blood transfusion. A double sandwich enzyme-linked immunosorbent assay (ELISA) was established to determine the level of IgA in serum of 22609 healthy blood donors and confirmed by gel immunosorbent assay (GIA). Meanwhile, anti-IgA was detected in the samples of IgA deficiency. Among 22 609 healthy blood donors, 14 cases of IgA deficiency were found, the proportion was 0.062% (1: 1615). Among them, 7 cases were confirmed as IgA content of 0. 05 mg / dL, and the proportion of total IgA deficiency was 0. 031% (1: 3230) in blood donors in Shanghai area. Two of the 14 IgA deficiency (IgA5 mg/dL) samples were identified as having anti-IgA deficiency and anti-IgA in 0.009% (1: 11305). The frequency of IgA deficiency in healthy blood donors in Shanghai is between Japanese and Caucasian people. However, there is still a potential risk of blood transfusion for IgA deficient people. Some measures should be taken to prevent and avoid the occurrence of allergic reaction to blood transfusion.
【学位授予单位】：华东师范大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R392.1
本文编号：2169187
[Abstract]:Immunoglobulin A (immunoglobulin A, IgA) deficiency generally refers to the IgA content in the serum is less than or absent from 5mg/dL, and when the content is lower than 0.05mg/dL, it is called IgA deficiency. Anti-IgA is produced in individuals with IgA deficiency, which increases the risk of allergic reactions to blood transfusion when infused with blood components containing IgA, and the frequency of shock or death in severe cases in different ethnic groups has been extensively studied. And there are differences. The incidence of IgA in healthy blood donors in China is still unknown, and there is no specific transfusion strategy for patients with IgA deficiency. In order to obtain the frequency of IgA deficiency in healthy blood donors of Han nationality from Shanghai and its surrounding areas, the risk of allergic reaction of IgA transfusion was preliminarily analyzed in order to ensure the safety of blood transfusion. A double sandwich enzyme-linked immunosorbent assay (ELISA) was established to determine the level of IgA in serum of 22609 healthy blood donors and confirmed by gel immunosorbent assay (GIA). Meanwhile, anti-IgA was detected in the samples of IgA deficiency. Among 22 609 healthy blood donors, 14 cases of IgA deficiency were found, the proportion was 0.062% (1: 1615). Among them, 7 cases were confirmed as IgA content of 0. 05 mg / dL, and the proportion of total IgA deficiency was 0. 031% (1: 3230) in blood donors in Shanghai area. Two of the 14 IgA deficiency (IgA5 mg/dL) samples were identified as having anti-IgA deficiency and anti-IgA in 0.009% (1: 11305). The frequency of IgA deficiency in healthy blood donors in Shanghai is between Japanese and Caucasian people. However, there is still a potential risk of blood transfusion for IgA deficient people. Some measures should be taken to prevent and avoid the occurrence of allergic reaction to blood transfusion.
【学位授予单位】：华东师范大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R392.1
【参考文献】
相关期刊论文 前4条
1 王可鹏,李薇,闫宏;IgA缺乏引起输血反应1例[J];齐齐哈尔医学院学报;2001年09期
2 陈秉宇;梅梦寒;谢一唯;;受血者输血前检测IgA含量的临床价值[J];检验医学;2009年12期
3 肖淑芝;一例IgA缺乏症患者输血致过敏性休克的急救及护理[J];天津护理;2004年04期
4 刘达庄;输血免疫反应预防(3)[J];外科理论与实践;2005年01期
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