MRSA大鼠感染性心内膜炎模型的建立

发布时间：2018-08-09 14:25

【摘要】：目的选用ATCC43300标准菌株,建立MRSA感染的大鼠心内膜炎模型。方法大鼠经右侧颈总动脉插管至左心室,术后24 h,尾静脉注射10~3、10~4和10~5 CFU/只感染量的ATCC43300菌液,分别在感染后的1、3、5和7 d处死大鼠,采集心脏赘生物、脾脏和肾脏进行菌落计数,同时设非手术感染组和手术非感染组作为对照。结果感染1 d后,心脏插管大鼠瓣膜处均有赘生物形成,严重者甚至形成壁性赘生物,且随感染菌浓度的增加,心内膜炎感染程度加剧。10~3 CFU/只感染量组、10~4 CFU/只感染量组、10~5 CFU/只感染量组,赘生物细菌计数的对数值分别为7.04±1.38、9.32±1.22、9.78±1.03;感染3 d后,细菌性心内膜炎感染最为严重,细菌计数的对数值最高可达10.03±0.71;随后的5~7 d,感染程度随感染量均有不同程度的下降,10~3 CFU/只感染量组和10~4 CFU/只感染量组,分别下降至6.87±0.63和8.23±1.05;脾脏和肾脏的感染趋势与心脏大致相同,但感染程度低于心脏。手术非感染组和非手术感染组则无赘生物的形成。结论应用该方法建立的模型具有可操作性强、简单、稳定、重复性好和成功率高的特点,更加适用于抗菌药物机制、新药筛选评价及心内膜炎相关性疾病的研究。
[Abstract]:Objective to establish the rat model of endocarditis infected with MRSA by using ATCC43300 standard strain. Methods the rats were intubated to the left ventricle through the right common carotid artery. After 24 hours of operation, the rats were injected into the tail vein with the amount of ATCC43300 bacteria only infected for 10 ~ 3 ~ 10 ~ (4) and 10 ~ (5) CFU/. The rats were killed on the 5th and 7th day after infection, and the cardiac neoplasm, spleen and kidney were collected for colony counting. At the same time, the non-operative infection group and the operative non-infection group were used as the control group. Results after 1 day of infection, there were vegetations in the valve of rats with cardiac catheterization, and even wall vegetations were formed in severe cases, and with the increase of the concentration of infected bacteria. The infection degree of endocarditis was increased. The infection rate of 10 ~ 4 CFU/ only group was 10 ~ 4 CFU/. The logarithmic values of bacterial count of neoplasm were 7.04 卤1.389.32 卤1.22 ~ 9.78 卤1.03.The bacterial endocarditis infection was the most serious after 3 days of infection. The maximum logarithmic value of bacterial count was 10.03 卤0.71, and the infection degree decreased with the infection amount of 10 ~ 3 CFU/ and 10 ~ 4 CFU/, respectively, to 6.87 卤0.63 and 8.23 卤1.05.The infection tendency of spleen and kidney was approximately the same as that of heart. But the degree of infection is lower than that of the heart. No neoplasm was found in the surgical non-infection group and non-operative infection group. Conclusion the model established by this method has the characteristics of strong maneuverability, simplicity, stability, good reproducibility and high success rate, and is more suitable for the study of antimicrobial mechanism, new drug screening and evaluation, and endocarditis related diseases.
【作者单位】：中国医学科学院北京协和医学院医药生物技术研究所药理室;
【基金】：“十二五”国家科技重大专项(2012ZX09301002-001、2012ZX09301002-005、2014ZX09507009-012) 北京市重点实验室科技创新基地培育与发展工程(Z141102004414065) 高等学校博士学科点专项科研基金(20121106120027)
【分类号】：R-332;R542.41

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