高果糖饮食诱导大鼠代谢综合征的模型研究

发布时间：2018-08-10 16:33

【摘要】：目的：建立高果糖饮食诱导大鼠代谢综合征模型。方法：将周龄为8周的雄性SD大鼠随机分成2组,对照组(n=15)喂饮普通净水,果糖组(n=33)喂饮15%果糖水,共8周。于喂饮果糖前后测定各组大鼠的体重、24小时饮食、12小时尿量、血压、空腹血糖和血液生化指标。喂饮果糖8周后,通过胰岛素耐量试验和高胰岛素-正葡萄糖钳夹技术比较各组大鼠胰岛素敏感性。结果：喂饮果糖导致动物出现了多饮多尿少食的现象。与对照组大鼠相比,果糖组大鼠在喂饮果糖6-8周后,产生了明显的高血压、高脂血症、胰岛素抵抗及高胰岛素血症等症状。8周的果糖喂饮并未导致果糖组大鼠出现明显的肥胖和高血糖,但ITT和糖钳实验证明,果糖组大鼠出现了明显的胰岛素抵抗。结论：成功建立高果糖饮食诱导大鼠代谢综合征模型,为进一步研究代谢综合征的病理生理机制和开发预防和治疗代谢综合征的药物提供基础。
[Abstract]:Objective: to establish a rat model of metabolic syndrome induced by high fructose diet. Methods: male Sprague-Dawley rats aged 8 weeks were randomly divided into two groups: the control group (n = 15) was fed with normal purified water, and the fructose group (n ~ (33) was fed with 15% fructose water for 8 weeks. Before and after drinking fructose, the body weight of rats in each group was measured, and the urine volume, blood pressure, fasting blood glucose and blood biochemical indexes were measured. After fed fructose for 8 weeks, insulin sensitivity was compared by insulin tolerance test and hyperinsulin-glucose clamp technique. Results: feeding fructose led animals to drink more urine and less food. Compared with the control group, the fructose group produced significant hypertension and hyperlipidemia after 6-8 weeks of drinking fructose. 8 weeks of fructose feeding with insulin resistance and hyperinsulinemia did not result in obvious obesity and hyperglycemia in fructose group, but ITT and glucose clamp experiments showed that fructose group had obvious insulin resistance. Conclusion: the rat model of metabolic syndrome induced by high fructose diet is successfully established, which provides a basis for further study on the pathophysiological mechanism of metabolic syndrome and the development of drugs for prevention and treatment of metabolic syndrome.
【学位授予单位】：华东师范大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R589.2;R-332

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