FTY720抑制小鼠植骨气囊模型中聚乙烯磨损颗粒诱导的骨溶解效应

发布时间：2018-08-12 15:56

【摘要】：目的:在小鼠植骨气囊动物模型中,观察外源性FTY720(Fingolimod,芬戈莫德)对骨溶解的抑制作用。方法:构建小鼠植骨气囊模型,将构建好气囊模型的小鼠分成4组:空白组(气囊内注入生理盐水、腹腔注射DMSO),FTY720组(气囊内注入生理盐水、腹腔注射FTY720),聚乙烯颗粒组(气囊内注入聚乙烯颗粒、腹腔注射DMSO),聚乙烯颗粒+FTY720组(气囊内注入聚乙烯颗粒、腹腔注射FTY720)。4周后处死小鼠后取气囊组织进行HE染色观察炎症反应和炎症细胞渗出情况,取组织匀浆行Elisa法检测气囊组织中IL-6和TNF-α浓度、取骨组织行电镜扫描观察骨片吸收情况,取组织匀浆行RT-PCR检测破骨细胞相关基因抗酒石酸酸性磷酸酶(TRAP)mRNA表达水平。结果:①HE染色后炎症细胞渗出量,聚乙烯颗粒+FTY720组(4892±457)与聚乙烯颗粒组(4931±572)比较无统计学差异(P0.05),FTY720组(2013±375)与空白组(2151±310)比较无统计学差异(P0.05);含有聚乙烯颗粒组高于无聚乙烯颗粒组(P0.05)。②Elisa法检测炎症因子(IL-6、TNF-α)浓度,聚乙烯颗粒+FTY720组〔(130.13±3.22)(、129.22±5.02)〕与聚乙烯颗粒组〔(149.22±6.09)(、140.52±2.68)〕比较无统计学差异(P0.05);FTY720组〔(68.24±2.83)、(82.88±5.34)〕与空白组〔(69.51±2.31)(、74.15±4.64)〕比较没有统计学差异(P0.05);含有聚乙烯颗粒组高于无聚乙烯颗粒组(P0.05)。③扫描电镜检测骨片吸收面积与视野面积百分比,聚乙烯颗粒+FTY720组〔(10.21±1.78)%〕低于聚乙烯颗粒组〔(17.79±2.56)%〕,FTY720组〔(1.82±1.37)%〕低于空白组〔(6.27±2.11)%〕;并且含有聚乙烯颗粒组高于无聚乙烯颗粒组(P0.05)。④破骨细胞相关基因TRAP mRNA表达量,聚乙烯颗粒+FTY720组(0.53±0.04)低于聚乙烯颗粒组(0.74±0.05),FTY720组(0.22±0.03)低于空白组(0.38±0.04);而含有聚乙烯颗粒组高于无聚乙烯颗粒组(P0.05)。结论:在小鼠植骨气囊动物模型中,FTY720能有效抑制聚乙烯颗粒诱导的局部骨溶解作用。
[Abstract]:Aim: to observe the inhibitory effect of exogenous FTY720 (Fingolimod) on osteolysis in mice. Methods: the mice were divided into 4 groups: blank group (intravesical injection of normal saline, intraperitoneal injection of DMSO) FTY720 group (intravesical injection of normal saline). FTY720 was injected intraperitoneally, polyethylene granules were injected into air bags and DMSO), polyethylene particles were injected intraperitoneally in FTY720 group. After 4 weeks of intraperitoneal injection of FTY720, the mice were sacrificed, and the inflammatory reaction and the exudation of inflammatory cells were observed by HE staining. The concentration of IL-6 and TNF- 伪 in the balloon tissue was detected by Elisa method, and the bone tissue was scanned by electron microscope to observe the absorption of bone slices. Tissue homogenate was used to detect the expression of tartrate-resistant acid phosphatase (TRAP) mRNA) in osteoclasts by RT-PCR. Results the amount of exudation of inflammatory cells after 1: 1HE staining, There was no significant difference between polyethylene granule FTY720 group (4892 卤457) and polyethylene granule group (4931 卤572) (P0.05). There was no statistical difference between FTY720 group (2013 卤375) and blank group (2151 卤310), and the concentration of inflammatory factor (IL-6 TNF- 伪) in PE particle group was higher than that in polyethylene particle group (P0.05). There was no significant difference between polyethylene granule FTY720 group (130.13 卤3.22) (129.22 卤5.02) and polyethylene granule group (149.22 卤6.09) (140.52 卤2.68) (P0.05). FTY720 group (68.24 卤2.83), (82.88 卤5.34) and blank group (69.51 卤2.31) (74.15 卤4.64) had no statistical difference (P0.05). The percentage of bone absorption area and visual field area was measured by scanning electron microscope. The TRAP mRNA expression of osteoclast associated genes in PE granule FTY720 group (10.21 卤1.78)% was lower than that in polyethylene granule group (17.79 卤2.56%) and in PE particle group (1.82 卤1.37)%, and in polyethylene particle group was higher than that in polyethylene particle group (P0.05). Polyethylene granule FTY720 group (0.53 卤0.04) was lower than polyethylene granule group (0.74 卤0.05), FTY720 group (0.22 卤0.03) was lower than blank group (0.38 卤0.04), and polyethylene particle group was higher than non-polyethylene granule group (P0.05). Conclusion: FTY720 can effectively inhibit the local osteolysis induced by polyethylene granules in mouse bone graft balloon animal model.
【作者单位】：重庆医科大学附属第一医院骨科;重庆医科大学附属第二医院骨科;
【基金】：国家自然科学基金(NSFC,81071490)
【分类号】：R687.4

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