丹参酮对人工关节无菌性松动小鼠气囊模型中炎症反应的影响
发布时间：2018-08-13 13:15
【摘要】：目的:研究丹参酮对人工关节无菌性松动小鼠气囊模型中炎症反应的影响。方法:将小鼠随机分为空白对照组(生理盐水)、钛颗粒组(生理盐水)和丹参酮低、中、高剂量组(50、100、200 mg/kg),每组10只。所有小鼠建立气囊模型,除空白对照组小鼠气囊内注入0.5 mL生理盐水外,其余各组小鼠气囊内均注入0.5 mL钛颗粒悬液(10 mg/mL),24 h后按0.1 mL/10 g连续ig相应药物14 d。末次给药24 h后收集气囊,肉眼和苏木精-伊红染色显微镜观察气囊炎症情况,计算炎症细胞密度;实时定量聚合酶链式反应法检测气囊组织中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)m RNA表达量;酶联免疫吸附法检测气囊组织中TNF-α、IL-1β蛋白表达量。结果:与空白对照组比较,钛颗粒组小鼠气囊红肿明显,可见较多渗出及新生血管,炎症反应严重,炎症细胞密度明显增加(P0.05);TNF-α、IL-1βm RNA及蛋白表达明显增强(P0.05)。与钛颗粒组比较,丹参酮各剂量组小鼠气囊红肿减轻,渗出及新生血管减少,炎症反应减轻,炎症细胞密度明显减小(P0.05);TNF-α、IL-1βm RNA及蛋白表达明显减弱(P0.05),且呈剂量依赖性。结论:丹参酮可有效抑制人工关节无菌性松动小鼠气囊模型中的无菌性炎症反应。
[Abstract]:Aim: to study the effect of tanshinone on inflammatory reaction in aseptic loosening mouse airbag model. Methods: the mice were randomly divided into control group (normal saline), titanium granule group (normal saline) and tanshinone low, medium and high dose group (50100200 mg/kg), each group had 10 rats. All the mice were injected with 0. 5 mL normal saline into the airbag of the control group, and 0. 5 mL titanium granule suspension (10 mg/mL) was injected into the airbag of all the mice for 24 h, and the corresponding drug was given orally for 14 days at the dose of 0. 1 mL/10 g. After the last administration for 24 hours, the air sacs were collected, and the inflammation was observed by naked eye and hematoxylin-eosin staining microscope, and the density of inflammatory cells was calculated. The expression of tumor necrosis factor- 伪 (TNF- 伪) and interleukin-1 尾 (IL-1 尾) m RNA) was detected by real-time quantitative polymerase chain reaction (RQPCR), and the expression of TNF- 伪 and IL-1 尾 was detected by enzyme-linked immunosorbent assay (Elisa). Results: compared with the blank control group, the mice in the titanium granule group showed obvious redness and swelling of the airbag, more exudation and neovascularization, severe inflammatory reaction, and obvious increase in the density of inflammatory cells (P0.05). The expression of IL-1 尾 m RNA and protein in the TNF- 伪 + IL-1 尾 m group was significantly increased (P0.05). Compared with the titanium granule group, the balloon redness, exudation and neovascularization, inflammatory reaction and the density of inflammatory cells decreased significantly in tanshinone group (P0.05), and the expression of IL-1 尾 m RNA and protein decreased significantly (P0.05) in a dose-dependent manner. Conclusion: tanshinone can effectively inhibit the aseptic inflammatory reaction in aseptic loosening mouse airbag model.
【作者单位】： 赣南医学院第一附属医院骨科;赣南医学院;
【分类号】：R285.5;R-332
本文编号：2181107
[Abstract]:Aim: to study the effect of tanshinone on inflammatory reaction in aseptic loosening mouse airbag model. Methods: the mice were randomly divided into control group (normal saline), titanium granule group (normal saline) and tanshinone low, medium and high dose group (50100200 mg/kg), each group had 10 rats. All the mice were injected with 0. 5 mL normal saline into the airbag of the control group, and 0. 5 mL titanium granule suspension (10 mg/mL) was injected into the airbag of all the mice for 24 h, and the corresponding drug was given orally for 14 days at the dose of 0. 1 mL/10 g. After the last administration for 24 hours, the air sacs were collected, and the inflammation was observed by naked eye and hematoxylin-eosin staining microscope, and the density of inflammatory cells was calculated. The expression of tumor necrosis factor- 伪 (TNF- 伪) and interleukin-1 尾 (IL-1 尾) m RNA) was detected by real-time quantitative polymerase chain reaction (RQPCR), and the expression of TNF- 伪 and IL-1 尾 was detected by enzyme-linked immunosorbent assay (Elisa). Results: compared with the blank control group, the mice in the titanium granule group showed obvious redness and swelling of the airbag, more exudation and neovascularization, severe inflammatory reaction, and obvious increase in the density of inflammatory cells (P0.05). The expression of IL-1 尾 m RNA and protein in the TNF- 伪 + IL-1 尾 m group was significantly increased (P0.05). Compared with the titanium granule group, the balloon redness, exudation and neovascularization, inflammatory reaction and the density of inflammatory cells decreased significantly in tanshinone group (P0.05), and the expression of IL-1 尾 m RNA and protein decreased significantly (P0.05) in a dose-dependent manner. Conclusion: tanshinone can effectively inhibit the aseptic inflammatory reaction in aseptic loosening mouse airbag model.
【作者单位】： 赣南医学院第一附属医院骨科;赣南医学院;
【分类号】：R285.5;R-332
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1 贾育松;中药淫羊藿苷预防人工髋关节无菌性松动实验研究[D];成都中医药大学;2008年
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