新疆中哈边境地区分离到远东型和西伯利亚型蜱传脑炎病毒

发布时间：2018-08-14 08:46

【摘要】：目的研究新疆中哈边境阿拉套山夏尔西里自然保护区蜱传脑炎疫源地病原的基因型及生物学特征。方法采用布旗法采集蜱,活蜱保存或液氮冻存;采用BALB/c小鼠与BHK-21细胞进行蜱传脑炎病毒分离培养;采用RT-PCR扩增蜱传脑炎病毒远东型FE和西伯利亚型S特异基因片段并测定其序列。结果从新疆中哈边境阿拉套山夏尔西里自然保护区全沟硬蜱和森林革蜱中分离出16株蜱传脑炎病毒株,通过对扩增基因序列比对分析,明确其中13株为远东型,3株为西伯利亚型。结论从新疆中哈边境阿拉套山夏尔西里自然保护区分离到远东型和西伯利亚型蜱传脑炎病毒,该地区为两种亚型病毒共存的蜱传脑炎自然疫源地。
[Abstract]:Objective to study the genotypes and biological characteristics of tick-borne encephalitis foci in Alatao Mountain, Xinjiang. Methods ticks were collected by cloth flag method, stored by living ticks or frozen by liquid nitrogen, and isolated and cultured by BALB/c mice and BHK-21 cells. RT-PCR was used to amplify and sequence the FFE and S specific genes of tick-borne encephalitis virus. Results Sixteen tick-borne encephalitis virus strains were isolated from Chalxi Nature Reserve in Alatao Mountain, Xinjiang, China. The results showed that 16 tick-borne encephalitis virus strains were analyzed by sequence alignment of amplified genes. 13 of them were of far East type and 3 of them were Siberia type. Conclusion far East and Siberian tick-borne encephalitis viruses were isolated from Alatao Mountain Sherri Nature Reserve in the Sino-Kazak border of Xinjiang. This area is the natural focus of tick-borne encephalitis in which two subtypes of viruses coexist.
【作者单位】：新疆军区疾病预防控制中心;阿拉山口出入境检验检疫局;
【基金】：国家自然科学基金项目(No.U1303104)~~
【分类号】：R373.31

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