祛痰活血颗粒对NAFLD模型大鼠脂肪组织AQP7,p38MAPK表达的影响

发布时间：2018-08-14 15:05

【摘要】：目的:探讨祛痰活血颗粒治疗非酒精性脂肪性肝病(NAFLD)大鼠的作用,并探讨其相关的作用机制。方法:40只SD大鼠随机分成正常组(6只),模型组(34只);除正常组外,采用高脂饮食诱导的方法诱导NAFLD大鼠模型,确认造模成功后,将剩余模型组(30只)随机分为5组,每组6只,分别为模型组,祛痰活血颗粒高、中、低剂量组(10,5,2.5 g·kg~(-1)),SB203580组(3 mg·kg~(-1)),分别予以灌胃祛痰活血颗粒及腹腔注射p38MAPK抑制剂SB203580,连续干预4周。苏木素-伊红(HE)及油红O染色观察肝脏病理变化;试剂盒测定血清甘油三酯(TG),总胆固醇(TC),天门冬氨酸氨基转移酶(AST),丙氨酸氨基转移酶(ALT),肝脏TG;实时荧光定量PCR(Real-time PCR)检测脂肪组织水通道蛋白7(AQP7)及p38分裂原激活的蛋白激酶(p38MAPK)mRNA表达,酶联免疫吸附测定(ELISA)法检测肝脏游离脂肪酸(FFA),脂肪组织AQP7和磷酸化p38MAPK(p-p38MAPK)的表达。结果:与正常组比较,模型组大鼠肝脏脂肪变性明显,血清TG,TC,AST,ALT和肝脏TG,FFA明显升高,脂肪组织中AQP7 mRNA及AQP7含量表达降低,p38MAPK mRNA及p-p38MAPK含量升高(P0.05);与模型组比较,祛痰活血颗粒和SB203580可减轻NAFLD大鼠肝脏脂肪变性程度;明显降低NAFLD大鼠的血清TG,TC,AST,ALT,肝脏TG,FFA(P0.05);各中药组和SB203580组大鼠脂肪组织AQP7 mRNA和AQP7含量表达明显升高,p38MAPK mRNA和pp38MAPK含量明显降低(P0.05)。结论:祛痰活血颗粒能减轻或者逆转肝脏的脂肪变性,其机制可能与抑制脂肪组织p38MAPK活化、上调AQP7表达,增加甘油入血代谢,减少进入肝脏的FFA,从而降低肝脏TG蓄积有关。
[Abstract]:Objective: to investigate the effect of Quphan Huoxue granule on (NAFLD) rats with non alcoholic fatty liver disease and its related mechanism. Methods 40 Sprague-Dawley rats were randomly divided into normal group (n = 6), model group (n = 34), NAFLD rat model induced by high-fat diet, and the remaining model group (n = 30) were randomly divided into 5 groups, 6 rats in each group. The model group was the model group with high and middle dose of expelling phlegm and activating blood granule, and the low dose group (10 ~ 5U 2.5 g kg ~ (-1) with SB203580 (3 mg kg ~ (-1),) was given by gavage of Quphan Huoxue granule and intraperitoneal injection of p38MAPK inhibitor SB203580 for 4 weeks. The pathological changes of liver were observed by hematoxylin eosin (HE) and oil red O staining. Serum triglyceride (TG), total cholesterol (TC), aspartate aminotransferase (AST), alanine aminotransferase (ALT), liver TGs, real-time quantitative PCR (Real-time PCR) detection of adipose tissue aquaporin 7 (AQP7) and p38 mitogen-activated protein kinase (p38MAPK) mRNA were detected. The expression of AQP7 and phosphorylated p38MAPK (p-p38MAPK) in adipose tissue of liver free fatty acid (FFA),) was detected by enzyme linked immunosorbent assay (ELISA). Results: compared with the normal group, the hepatic steatosis of the model group was significantly higher, the serum TGG tctastit and liver TGP FFA were significantly increased, and the expression of AQP7 mRNA and AQP7 in the adipose tissue decreased significantly (P0.05), and compared with the model group, the expression of p38 MAPK mRNA and p-p38MAPK increased significantly (P0.05), and compared with the model group, the expression of AQP7 and AQP7 mRNA in the adipose tissue decreased significantly (P0.05). Quphan Huoxue granule and SB203580 could reduce the degree of hepatic steatosis in NAFLD rats; significantly reduce the serum TGG TCCA alt and liver TGN FFA in NAFLD rats (P0.05); the expression of AQP7 mRNA and AQP7 in adipose tissue of rats in Chinese medicine group and SB203580 group were significantly increased (P0.05). 3. The expression of AQP7 mRNA and AQP7 in adipose tissue of rats in each Chinese medicine group and SB203580 group were significantly increased (P0.05). Conclusion: Quphan Huoxue granule can attenuate or reverse fatty degeneration of liver, and its mechanism may be related to inhibition of p38MAPK activation in adipose tissue, upregulation of AQP7 expression, increase of blood metabolism of glycerol, decrease of FFAs entering into liver and decrease of accumulation of TG in liver.
【作者单位】：西南医科大学附属中医医院;
【基金】：四川省教育厅2014年度理科重点项目(14ZA0144) 四川省教育厅2015年度理科一般项目(15ZB0156) 泸州市科技局2016年度项目[2016-R-70(23/24)]
【分类号】：R285.5;R-332

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