芎归六君子汤对慢性阻塞性肺疾病气虚血瘀痰阻证模型大鼠气道Muc5ac水平的影响
发布时间：2018-08-15 17:33
【摘要】：目的观察芎归六君子汤对慢性阻塞性肺疾病(COPD)气虚血瘀痰阻证模型大鼠气道Muc5ac水平的影响。方法60只大鼠按随机数字表随机分为空白组、芎归六君子汤自身对照组、模型组、芎归六君子汤干预组(低、中、高剂量),每组10只。采用改良的香烟烟熏和脂多糖气管注入法联合制备模型。采用芎归六君子汤干预15天后取材,观察肺泡灌洗液(BALF)中Muc5ac水平、气道上皮Muc5ac蛋白表达及肺组织Muc5ac mRNA表达。结果与空白组比较,模型组大鼠BALF中的Muc5ac水平及气道上皮Muc5ac蛋白的表达增加,肺组织Muc5ac mRNA表达上调(P0.05)。芎归六君子汤能降低COPD气虚血瘀痰阻证模型大鼠BALF中的Muc5ac水平、气道上皮Muc5ac蛋白表达,下调肺组织Muc5ac mRNA表达,且以高剂量组较为明显(P0.05)。结论芎归六君子汤在一定程度上降低COPD气虚血瘀痰阻证模型大鼠气道Muc5ac水平,减少气道黏液高分泌。
[Abstract]:Objective to observe the effect of Xionggui Liujunzi decoction on the level of airway Muc5ac in (COPD) rats with Qi deficiency and blood stasis and phlegm blocking syndrome. Methods Sixty rats were randomly divided into blank group, control group, model group and intervention group (low, medium and high dosage) with 10 rats in each group. The model was prepared by modified cigarette fumigation and endotracheal injection of lipopolysaccharide (LPS). After treated with Xionggui Liujunzi decoction for 15 days, the levels of Muc5ac in alveolar lavage fluid (BALF), the expression of Muc5ac protein in airway epithelium and the expression of Muc5ac mRNA in lung tissue were observed. Results compared with the blank group, the level of Muc5ac in BALF and the expression of Muc5ac protein in airway epithelium were increased in the model group, and the expression of Muc5ac mRNA was up-regulated in the lung tissue (P0.05). Xionggui Liujunzi decoction could reduce the level of Muc5ac in BALF, Muc5ac protein expression in airway epithelium and Muc5ac mRNA expression in lung tissue of COPD rats with Qi deficiency and blood stasis and phlegm obstruction, especially in high dose group (P0.05). Conclusion Xionggui Liujunzi decoction can reduce the level of airway Muc5ac and reduce airway mucus hypersecretion in COPD rats with Qi deficiency and blood stasis and phlegm obstruction to some extent.
【作者单位】： 广西中医药大学第一附属医院;
【基金】：国家自然科学基金(No.81360534) 广西自然科学基金(No.2015GXNSFBA139123)
【分类号】：R285.5;R-332
，
本文编号：2184922
[Abstract]:Objective to observe the effect of Xionggui Liujunzi decoction on the level of airway Muc5ac in (COPD) rats with Qi deficiency and blood stasis and phlegm blocking syndrome. Methods Sixty rats were randomly divided into blank group, control group, model group and intervention group (low, medium and high dosage) with 10 rats in each group. The model was prepared by modified cigarette fumigation and endotracheal injection of lipopolysaccharide (LPS). After treated with Xionggui Liujunzi decoction for 15 days, the levels of Muc5ac in alveolar lavage fluid (BALF), the expression of Muc5ac protein in airway epithelium and the expression of Muc5ac mRNA in lung tissue were observed. Results compared with the blank group, the level of Muc5ac in BALF and the expression of Muc5ac protein in airway epithelium were increased in the model group, and the expression of Muc5ac mRNA was up-regulated in the lung tissue (P0.05). Xionggui Liujunzi decoction could reduce the level of Muc5ac in BALF, Muc5ac protein expression in airway epithelium and Muc5ac mRNA expression in lung tissue of COPD rats with Qi deficiency and blood stasis and phlegm obstruction, especially in high dose group (P0.05). Conclusion Xionggui Liujunzi decoction can reduce the level of airway Muc5ac and reduce airway mucus hypersecretion in COPD rats with Qi deficiency and blood stasis and phlegm obstruction to some extent.
【作者单位】： 广西中医药大学第一附属医院;
【基金】：国家自然科学基金(No.81360534) 广西自然科学基金(No.2015GXNSFBA139123)
【分类号】：R285.5;R-332
，
本文编号：2184922
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