脾虚痰湿型肥胖糖尿病胰岛素抵抗大鼠病证结合模型的建立

发布时间：2018-08-16 09:37

【摘要】：目的:建立一种病证结合的脾虚痰湿型肥胖糖尿病胰岛素抵抗大鼠模型。方法:60只SD大鼠按体质量随机分为正常对照(A)组和模型组,模型组又分为肥胖糖尿病(B)组、脾虚痰湿型肥胖糖尿病(C)组和脾虚痰湿肥胖糖尿病加中药健脾化湿方治疗(D)组。模型组复制肥胖糖尿病胰岛素抵抗大鼠疾病模型,C组和D组复制脾虚痰湿型肥胖糖尿病胰岛素抵抗大鼠模型,D组给予健脾化湿方干预4周。观察各组大鼠的一般行为学变化、毛色,大便、体质量、摄食量、饮水量、肛温、游泳耐力、计算脾虚积分;测定大鼠的空腹血糖(FPG)、空腹胰岛素(Fins),计算胰岛素抵抗指数(HOMA-IR)、胰岛素敏感指数(ISI);口服葡萄糖耐量试验(OGTT)、总胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、游离脂肪酸(FFA);观察肝脏病理变化。并采用健脾化湿方进行反证。结果:高脂饲料喂养8周后,模型组平均体质量超过A组(P0.05);STZ注射72h后,模型组大鼠FPG、Fins、HOMA-IR明显高于A组(P0.05),ISI明显低于A组(P0.01);模型组大鼠OGTT各点血糖值均高于A组(P0.05)。经过4周脾虚痰湿阶段造模后,大鼠表现为体质量下降、饮食量、饮水量明显减少,游泳耐力显著下降,被毛油腻,倦怠,懒动,蜷缩扎堆,大便溏,脾虚积分显著增加(P0.05,P0.01),肛温无明显变化;TC、LDL-C、FFA升高(P0.05,P0.01)。经过中药健脾化湿方干预后,大鼠出现体质量下降,饮食量、饮水量下降,游泳耐力增加,倦怠、乏力,大便溏症状缓解,脾虚积分下降(P0.05,P0.01);大鼠FPG、Fins、HOMA-IR、ISI明显改善(P0.05,P0.01);血脂中TC、TG、LDL-C、FFA降低(P0.05,P0.01);肝脏病理结果显示,B、C组伴有不同程度的脂肪肝,D组脂肪肝程度减轻。结论:采用高脂饮食联合小剂量STZ可复制出肥胖糖尿病胰岛素抵抗大鼠疾病模型,采用饮食不节+劳倦过度+苦寒攻下复合法可成功建立脾虚痰湿型大鼠证候模型。
[Abstract]:Objective: to establish a model of insulin resistance in obese diabetic rats with spleen deficiency and phlegm dampness combined with disease and syndrome. Methods Sixty Sprague-Dawley rats were randomly divided into normal control (A) group and model group according to their body weight. The model group was divided into obese diabetic (B) group, spleen deficiency phlegm dampness type obesity diabetes (C) group and spleen deficiency phlegm dampness obesity diabetes mellitus plus traditional Chinese medicine Jianpi Hua Shen Fang treatment (D) group. The model group (group C) and group D (group D) were treated with Jianpi Huazheng recipe for 4 weeks. The changes of general behavior, hair color, stool, body weight, food intake, drinking water, anus temperature, swimming endurance and spleen deficiency score were observed. Insulin resistance index (HOMA-IR), insulin sensitivity index (ISI);), total cholesterol (OGTT), triglyceride (TC), (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein (LDL) were measured in rats with fasting blood glucose (FPG),) and fasting insulin (Fins),). Insulin sensitivity index (ISI);) was measured by oral glucose tolerance test (ISI);). LDL-C and free fatty acid (FFA); were used to observe the pathological changes of liver. And the use of invigorating spleen and dampness prescription to counter the evidence. Results: after feeding with high fat diet for 8 weeks, the average body mass of model group was higher than that of group A (P0.05) 72 hours after injection of STZ, the HOMA-IR of model group was significantly higher than that of group A (P0.05) and the blood glucose level of OGTT of model group was higher than that of group A (P0.05). After 4 weeks of spleen deficiency and phlegm dampness stage, the rats showed decreased body mass, decreased diet, drinking water, decreased swimming stamina, wool greasy, burnout, laziness, curling up and loose stools. The spleen deficiency score increased significantly (P0.05U P0.01), but the anal temperature did not change significantly (P0.05U P0.01). After the intervention of traditional Chinese medicine to invigorate spleen and remove dampness, the rats appeared body weight decline, diet quantity, drinking water decreased, swimming stamina increased, fatigue, loose stool symptoms alleviated. The scores of spleen deficiency (P0.05), HOMA-IRISI (P0.05 + P0.01), LDL-CfFA (P0.05P0.01) and liver pathological results showed that the degree of fatty liver was decreased in group D with different degrees of fatty liver. Conclusion: high fat diet combined with low dose STZ can reproduce the model of insulin resistance in obese diabetic rats, and the syndrome model of spleen deficiency and phlegm dampness type can be successfully established by the combination of diet, fatigue, excessive bitterness and cold attack.
【作者单位】：中山大学附属第一医院中医科;南方医科大学珠江医院中医科;
【基金】：国家自然科学基金项目(No.81302877) 广东省科技计划项目(No.2014A020212056) 广东省中医药局基金项目(No.20141057)~~
【分类号】：R259;R-332

【相似文献】