雌激素对绝经后不同鼠龄大鼠血清一氧化氮水平的影响

发布时间：2018-08-16 15:18

【摘要】：目的本实验研究通过建立不同鼠龄绝经模型,观察雌激素对绝经后不同鼠龄大鼠血清一氧化氮(NO)含量、主动脉内皮型一氧化氮合酶(eNOS) mRNA表达的影响以及主动脉组织形态学的变化。方法将青年(3月龄)雌性wistar大鼠30只和老年(18-20月龄)雌性wistar大鼠30只分别随机分为三组,每组10只：假手术组(Sham组)、去卵巢组(OVX组)、去卵巢+苯甲酸雌二醇补充组(OVE组)。Sham组：假手术+每3天肌注0.04m1生理盐水；OVX组：去除双侧卵巢+每3天肌注0.04m1生理盐水；OVE组：去除双侧卵巢+每3天肌注10ug苯甲酸雌二醇,4个月后处死大鼠并测定各组血清NO的水平、主动脉eNOS mRNA的表达并观察主动脉的组织形态学变化。结果1.青年Sham组,OVX组,OVE组的血清NO水平分别明显高于老年Sham组,OVX组,OVE组的血清NO水平,p0.05,p0.05,p0.05。去卵巢后,青年大鼠OVX组的血清NO水平明显低于Sham组,p0.05,但是在老年OVX组无明显差异。补充雌激素后,青年、老年OVE组的血清NO水平较OVX组明显增加,p0.05,p0.05。2.青年Sham组、OVE组主动脉eNOS mRNA的表达分别高于老年Sham组、OVE组,p0.05,p0.05。但是青年OVX组与老年OVX组主动脉eNOS mRNA的表达无明显差异,p=0.462。去卵巢后青年OVX组主动脉eNOS mRNA的表达明显显低于Sham组,p0.05；补充雌激素后,青年OVE组主动脉eNOS mRNA的表达较OVX组明显增加,p0.05。老年大鼠Sham组,OVX组,OVE组之间主动脉eNOS mRNA的表达无明显差异,p=0.579。结论1.在青年绝经后大鼠,雌激素治疗后可能通过上调内皮型一氧化氮合酶(eNOS) mRNA的表达来增加血清一氧化氮(NO)的水平。2.在老年绝经大鼠,雌激素治疗后增加血清一氧化氮(NO)的合成不是通过上调内皮型一氧化氮合酶(eNOS) mRNA的表达来实现。3.随着年龄增加,血清一氧化氮(NO)水平和内皮型一氧化氮合酶(eNOS) mRNA的表达都逐渐减少。
[Abstract]:Objective to study the effect of estrogen on serum nitric oxide (NO) content in postmenopausal rats by establishing menopausal models of different ages. Effects of aortic endothelial nitric oxide synthase (eNOS) mRNA) expression and changes of aortic histomorphology. Methods Thirty young (3-month-old) female wistar rats and 30 aged (18-20 months) female wistar rats were randomly divided into three groups. There were 10 rats in each group: sham operation group (Sham group), ovariectomized group (OVX group), ovariectomized estradiol benzoate supplement group (OVE group) .Sham group: sham group was given 0.04m1 saline every 3 days; In OVE group, 10ug estradiol benzoate was injected intramuscularly every 3 days after removal of bilateral ovaries. After 4 months, the rats were killed and serum no levels were measured. The expression of eNOS mRNA in aorta and the histomorphologic changes of aorta were observed. Result 1. The serum no level of OVX group in young Sham group was significantly higher than that of OVX group in elderly Sham group. After ovariectomy, the serum no level in OVX group was significantly lower than that in Sham group (p 0.05), but there was no significant difference in aged OVX group. After estrogen supplementation, the serum no level in the young and elderly OVE group was significantly higher than that in the OVX group. The expression of aortic eNOS mRNA in the young Sham group was higher than that in the elderly Sham group. However, there was no significant difference in the expression of aortic eNOS mRNA between the young OVX group and the old OVX group. After ovariectomized, the expression of aortic eNOS mRNA in the young OVX group was significantly lower than that in the Sham group (p 0.05), and after estrogen supplementation, the expression of eNOS mRNA in the aorta of the young OVE group was significantly higher than that in the OVX group. There was no significant difference in the expression of eNOS mRNA in aorta between OVX group and OVX group in aged rats. Conclusion 1. In young postmenopausal rats, estrogen treatment may increase the level of serum nitric oxide (NO) by up-regulating the expression of endothelial nitric oxide synthase (eNOS) mRNA). In elderly postmenopausal rats, the increase in serum nitric oxide (NO) synthesis after estrogen therapy is not achieved by up-regulating the expression of endothelial nitric oxide synthase (eNOS) mRNA). The level of serum nitric oxide (NO) and the expression of endothelial nitric oxide synthase (eNOS) mRNA) decreased with age.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R-332

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