双歧杆菌、乳酸杆菌在SD大鼠肠道动力研究

发布时间：2018-08-18 09:32

【摘要】：目的探讨双歧杆菌与乳酸杆菌在SD大鼠的肠道动力及肥胖对其影响. 方法利用羧基荧光素二乙酸琥珀酰亚胺酯(CFDA-SE)分子探针标记双歧杆菌、乳酸杆菌,以每只4ml×109CFU/ml灌喂肥胖及普饲组SD大鼠,分别于灌喂后0,2,4,8,12,24,48,72,96小时取大便,灌喂2,4,8,12,24小时取近端小肠、中段小肠、远端小肠、结肠等肠段,在荧光显微镜下观察标记菌数量。结果 1.肥胖组双歧杆菌2、4、24 h,乳酸杆菌2、12、24 h,大便细菌排出量较普饲组低(P0.05)； 2.双歧杆菌在肥胖组4h,普饲组2、4、8、24 h,大便细菌排出量与乳酸杆菌不同(P0.05)； 3.肥胖组双歧杆菌近端小肠在2、12 h,中段小肠在2、8 h,远端小肠在2、4、8、12h,结肠在2、4、12、24 h细菌在肠道的分布与普饲组不同(P0.05)； 4.肥胖组乳酸杆菌中段小肠在2h,远端小肠在8、12、24 h,结肠在2、12、24 h与普饲组不同(P0.05). 结论 1.双歧杆菌、乳酸杆菌在大鼠肠道动力不同：双歧杆菌较乳酸杆菌肠道运动更快,大便细菌排出迅速；双岐杆菌主要密集在结肠,而乳酸杆菌则能长时间停留在远端小肠及结肠； 2.肥胖对双歧杆菌、乳酸杆菌的肠道动力有一定的影响：肥胖延缓细菌的肠道运动,使细菌在小肠内长时间停留,大便细菌排出延迟,高峰推后,细菌排出波改变,排出量减少。
[Abstract]:Objective to investigate the intestinal motility of Bifidobacterium and Lactobacillus in SD rats and the effect of obesity. Methods Bifidobacterium and Lactobacillus were labeled with carboxyl fluorescein disuccinimide (CFDA-SE) molecular probe. Obese SD rats and SD rats were fed with 4ml 脳 109CFU/ml for 7296 hours. The proximal small intestine, middle small intestine, distal small intestine, colon and other intestinal segments were taken for 24 hours by perfusion. The number of labeled bacteria was observed under fluorescence microscope. Result 1. The excretion of fecal bacteria in obesity group was lower than that in general feeding group (P0.05). Bifidobacterium excretion was different from that of Lactobacillus in obesity group for 4 h and in general feeding group for 824 h (P0.05). The distribution of bacteria in the proximal small intestine of obesity group was different from that in the general feeding group (P0.05), the distribution of bacteria in the proximal small intestine was 2h, the middle intestine was 2h, the distal intestine was 812h, and the colon was 24 h. The middle small intestine of the obese group was at 2 hours, the distal small intestine was at 812 hours, and the colon was at 21: 12 hours, which was different from that of the general feeding group (P0.05). Conclusion 1. Bifidobacterium and Lactobacillus have different intestinal motility in rats: bifidobacterium moves faster than Lactobacillus and defecate bacteria excrete rapidly, bifidobacterium is mainly concentrated in colon, while Lactobacillus can stay in distal intestine and colon for a long time. 2. Obesity has a certain effect on the intestinal motility of bifidobacterium and lactobacillus: obesity delays the intestinal movement of bacteria, makes bacteria stay in the small intestine for a long time, delays fecal bacteria excretion, delays the peak, changes the bacterial excretion wave and reduces the excretion quantity.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R371

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