Actemra在大鼠肺动脉高压模型中对IL-6和survivin影响的研究

发布时间：2018-08-18 11:32

【摘要】：目的建立野百合碱（MCT）诱导大鼠肺动脉高压（PAH）动物模型，观测actemra对该模型肺动脉压、血清IL-6、survivin的影响，探讨其作用机制。方法 1、分组方法：实验动物大鼠按体重随机分为三组： A.空白对照组（n=10）；B.实验对照组（n=10）；C. actemra干预组（n=10）。A组在第一天腹腔注射0.9%氯化钠注射液60mg/kg；B组在第一天腹腔注射MCT60mg/kg；C组在第一天腹腔注射MCT60mg/kg和actemra：5mg/kg。 2、右心微导管测各组大鼠平均肺动脉压（mPAP）。 3、HE染色观察各组大鼠肺组织标本的血管壁增厚情况。 4、免疫组化观察IL-6和survivin在肺血管的表达情况。 5、ELISA法定量测定血清IL-6和survivin的含量，综合评估actemra对MCT诱导PAH大鼠模型的影响。结果 1、大鼠mPAP：Actemra干预组、空白对照组和实验对照组比较为（23.56±4.86）mmHg、（19.14±4.30）mmHg比（41.71±8.79）mmHg，p均0.05。Actemra干预组和空白对照组比较无统计学意义，p0.05。 2、HE染色显示实验对照组肺动脉血管壁明显增厚，周围有炎性细胞浸润，肺泡间隔明显增厚。Actemra干预组肺动脉血管内膜轻度水肿，血管壁轻度增厚。 3、免疫组化显示IL-6和survivin在实验对照组大鼠肺组织强表达，在Actemra干预组大鼠肺组织少量表达，而空白对照组大鼠肺组织不表达。 4、ELISA定量测定IL-6含量：Actemra干预组和空白对照组明显低于实验对照组，，组间比较为（1576.63±296.19）pg/ml、（1316.9798±71.20）pg/ml比（2069.49±295.60）pg/ml，p均0.05。Survivin含量：Actemra干预组和空白对照组明显低于实验对照组，组间比较为（12.22±1.11）ng/ml、（2.40±3.62）ng/ml比（43.32±8.16）ng/ml，p均0.05。结论 1、Actemra可有效抑制MCT诱导PAH的发生。 2、MCT诱导PAH产生可能与IL-6信号转导通路有关。
[Abstract]:Objective to establish an animal model of monocrotaline (MCT) induced pulmonary hypertension (PAH) in rats, observe the effect of actemra on pulmonary artery pressure and serum IL-6 survivin, and explore its mechanism. Methods 1. Group method: experimental rats were randomly divided into three groups according to their body weight: A. Control group (n = 10). The experimental control group (n = 10). Actemra intervention group (n = 10). Group A was intraperitoneally injected with 0.9% sodium chloride injection 60 mg / kg / kg on the first day, group B was intraperitoneally injected with 60 mg / kg MCT60 mg / kg on the first day. On the first day, MCT60mg/kg and actemraw 5 mg / kg 路2 were injected intraperitoneally in group C, and the mean pulmonary artery pressure (mPAP). _ 3) in each group was measured by right heart microcatheter. The expression of IL-6 and survivin in pulmonary vessels was observed by immunohistochemical staining. The serum IL-6 and survivin levels were measured by Elisa. To evaluate the effect of actemra on PAH rat model induced by MCT. Results 1. Rats in mPAP:Actemra intervention group, The difference between the blank control group and the experimental control group was (23.56 卤4.86) mmHg, (19.14 卤4.30) mmHg compared with (41.71 卤8.79) mmHgP in the 0.05.Actemra intervention group and the blank control group. The results of HE staining showed that the pulmonary artery wall of the control group was thicker and the inflammatory cells infiltrated around the pulmonary artery. Actemra intervention group showed slight edema of pulmonary artery intima and slight thickening of vascular wall. Immunohistochemical staining showed that IL-6 and survivin were strongly expressed in lung tissue of experimental control group and a little in Actemra intervention group. However, there was no expression in lung tissue of rats in blank control group. 4 the quantitative determination of IL-6 content by Elisa in intervention group and blank control group was significantly lower than that in experimental control group. The mean 0.05.Survivin content of 0.05.Survivin: Actemra intervention group and blank control group were (1576.63 卤296.19) PG / ml, (1316.9798 卤71.20) pg/ml vs (2069.49 卤295.60) PG / ml, (12.22 卤1.11) ng / ml, (2.40 卤3.62) ng/ml / (43.32 卤8.16) ng / ml, respectively. Conclusion 1 Actemra can effectively inhibit the occurrence of PAH induced by MCT. 2MCT-induced PAH may be related to IL-6 signal transduction pathway.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R-332

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