δ阿片受体激动剂对神经元慢性缺氧损伤的保护作用
发布时间：2018-08-18 15:14
【摘要】：目的通过建立大鼠慢性缺氧模型,观察不同缺氧时间及应用δ阿片受体激动剂下大鼠左皮层脑组织中Caspase-3的基因表达含量及相应的蛋白含量变化。 方法将54只SD大鼠随机分为3组,分为缺氧1天组,缺氧5天组,缺氧10天组,各组中又分为3个小组,即:对照组(C),缺氧组(H)及缺氧+给药组(UFP-512)(H+D)。断头取脑后取得大鼠左皮层脑组织,以β-actin为内参,利用RT-PCR及Western blotting实验测定所取组织中的Caspase-3的基因表达含量及相应的蛋白含量,通过统计分析方法比较组内指标的差异性。 结果随着缺氧时间的延长,组织中的Caspase-3基因表达含量及相应的蛋白含量增加,差异具有统计学意义(P0.05);缺氧1天组,Caspase-3基因表达含量及相应的蛋白含量变化不明显,组内比较,差异无统计学意义(P0.05);缺氧5天组,缺氧组及缺氧+给药组(UFP-512)的Caspase-3基因表达含量及相应的蛋白含量与对照组两两比较,差异具有统计学意义(P0.05),并且缺氧+给药组的Caspase-3基因表达含量及相应的蛋白含量较缺氧组低,差异同样具有统计学意义(P0.05);缺氧10天组的缺氧组及缺氧+给药组(UFP-512)的Caspase-3基因表达含量及相应的蛋白含量与对照组两两比较,差异具有统计学意义(P0.05),并且缺氧+给药组的Caspase-3基因表达含量及相应的蛋白含量与缺氧组比较,差异明显,仍具有统计学意义(P0.05)。 结论Caspase-3可以作为慢性缺氧所致神经元损伤程度的指标,而UFP-512作为一种新型的δ阿片受体激动剂通过激活δ阿片受体而减少Caspase-3的表达,起到一定脑保护作用。
[Abstract]:Objective to establish a rat model of chronic hypoxia and to observe the changes of Caspase-3 gene expression and protein content in the left cortical brain of rats under different hypoxia time and 未 opioid receptor agonist (未 opioid receptor agonist). Methods Fifty-four SD rats were randomly divided into three groups: hypoxia for one day, hypoxia for 5 days and hypoxia for 10 days. Each group was divided into three groups: control group, (C), hypoxia group, (H) group and hypoxia administration group (UFP-512) (H D). The left cortical brain tissue of rats was obtained after decapitation. 尾 -actin was used as the internal reference. The Caspase-3 gene expression and the corresponding protein content were measured by RT-PCR and Western blotting experiments. The differences of the indexes in the group were compared by statistical analysis. Results with the prolongation of hypoxia time, the expression of Caspase-3 gene and the corresponding protein content in tissues increased significantly (P0.05), but the expression of Caspase-3 gene and the corresponding protein content in hypoxia group were not significantly changed after 1 day of hypoxia, but there was no significant difference in the expression of Caspase-3 gene and the corresponding protein content between the two groups. There was no significant difference (P0.05). The expression of Caspase-3 gene and protein in hypoxia group, hypoxia group and hypoxia administration group (UFP-512) were compared with those in control group. The difference was statistically significant (P0.05), and the expression of Caspase-3 gene and the corresponding protein content in hypoxia group were lower than those in hypoxia group. The difference was also statistically significant (P0.05). The expression of Caspase-3 gene and the corresponding protein content in hypoxia group and UFP-512 group were compared with those in control group. The difference was statistically significant (P0.05), and the expression of Caspase-3 gene and the corresponding protein content in hypoxia group were significantly higher than those in hypoxia group (P0.05). Conclusion Caspase-3 can be used as a marker of neuron damage induced by chronic hypoxia, and UFP-512, as a new type of 未 opioid receptor agonist, can reduce the expression of Caspase-3 by activating 未 opioid receptor, and play a protective role in brain.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363
[Abstract]:Objective to establish a rat model of chronic hypoxia and to observe the changes of Caspase-3 gene expression and protein content in the left cortical brain of rats under different hypoxia time and 未 opioid receptor agonist (未 opioid receptor agonist). Methods Fifty-four SD rats were randomly divided into three groups: hypoxia for one day, hypoxia for 5 days and hypoxia for 10 days. Each group was divided into three groups: control group, (C), hypoxia group, (H) group and hypoxia administration group (UFP-512) (H D). The left cortical brain tissue of rats was obtained after decapitation. 尾 -actin was used as the internal reference. The Caspase-3 gene expression and the corresponding protein content were measured by RT-PCR and Western blotting experiments. The differences of the indexes in the group were compared by statistical analysis. Results with the prolongation of hypoxia time, the expression of Caspase-3 gene and the corresponding protein content in tissues increased significantly (P0.05), but the expression of Caspase-3 gene and the corresponding protein content in hypoxia group were not significantly changed after 1 day of hypoxia, but there was no significant difference in the expression of Caspase-3 gene and the corresponding protein content between the two groups. There was no significant difference (P0.05). The expression of Caspase-3 gene and protein in hypoxia group, hypoxia group and hypoxia administration group (UFP-512) were compared with those in control group. The difference was statistically significant (P0.05), and the expression of Caspase-3 gene and the corresponding protein content in hypoxia group were lower than those in hypoxia group. The difference was also statistically significant (P0.05). The expression of Caspase-3 gene and the corresponding protein content in hypoxia group and UFP-512 group were compared with those in control group. The difference was statistically significant (P0.05), and the expression of Caspase-3 gene and the corresponding protein content in hypoxia group were significantly higher than those in hypoxia group (P0.05). Conclusion Caspase-3 can be used as a marker of neuron damage induced by chronic hypoxia, and UFP-512, as a new type of 未 opioid receptor agonist, can reduce the expression of Caspase-3 by activating 未 opioid receptor, and play a protective role in brain.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363
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