纳米山药多糖结肠靶向胶囊对急性肝衰竭模型大鼠肠道菌群影响研究

发布时间：2018-08-19 06:54

【摘要】：目的评价研究纳米山药多糖对急性肝衰竭模型大鼠肠道菌群的影响。方法以硫代乙酰胺(TAA)腹腔注射造成大鼠肝衰竭并伴有肠道菌群失调模型,采用平板活菌计数法,检测造模前后大鼠的肠道菌落数量变化,判定模型是否成功,将大鼠随机分成正常对照组、纳米山药多糖组、丽珠肠乐组、自然恢复组。菌落计数法检测各组动物菌群数量,菌群易位,测定谷丙转氨酶(ALT)、总胆红素(TBIL)和肝脏指数。结果模型组与正常组比,双歧杆菌、乳酸杆菌数量下降,肠球菌、肠杆菌、类杆菌数量明显并出现菌群易位,肠道菌群失调模型建立。纳米山药多糖组与自然恢复组比可以显著抑制TAA引起的ALT、TBIL活性的升高,并降低大鼠肝脏指数,显著改善菌群失调和细菌易位情况。结论纳米山药多糖有改善急性肝衰竭模型大鼠菌群失调和细菌易位作用。
[Abstract]:Objective to evaluate the effect of nanometer yam polysaccharides on intestinal flora in rats with acute liver failure. Methods the rat model of hepatic failure with intestinal microflora disorder was induced by intraperitoneal injection of thioacetamide (TAA). The number of intestinal colonies of rats before and after the establishment of the model was determined by the method of plate count of live bacteria, and the success of the model was determined. Rats were randomly divided into normal control group, nanometer yam polysaccharide group, Lizhu Changle group and natural recovery group. The number, translocation, total bilirubin (TBIL) and liver index of alanine aminotransferase (ALT),) were measured by colony counting method. Results compared with the normal group, the number of Bifidobacterium and Lactobacillus decreased, the number of Enterococcus, Enterobacter and Bacteroid bacteria were obvious and translocation, and the intestinal flora imbalance model was established. Compared with the natural recovery group, nanometer yam polysaccharide group could significantly inhibit the increase of alt TBIL activity induced by TAA, decrease the liver index of rats, and improve the bacteriological imbalance and bacterial translocation. Conclusion nanometer yam polysaccharides can improve the bacteriological imbalance and bacterial translocation in rats with acute liver failure.
【作者单位】：佳木斯大学药学院;佳木斯大学基础医学院;
【基金】：国家自然科学基金(No.81274101)
【分类号】：R285.5;R-332

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