工频磁场对中国仓鼠肺成纤维细胞自噬的诱导作用
发布时间：2018-08-20 10:33
【摘要】：目的：随着电力事业的发展,人群接触极低频磁场的时间和强度愈来愈大,人们对极低频磁场的效应也愈加关注。早期的研究表明极低频磁场可以诱导细胞分子水平的改变,如：细胞缝隙连接功能(GJIC)下降、细胞膜表皮生长因子受体(EGFR)和肿瘤坏死因子受体(TNFR)聚簇,以及基因和蛋白质表达谱发生改变等等。细胞自噬是机体重要的一种防御和保护机制,它大量降解并重复利用长寿命蛋白质、大分子物质以及细胞内受损的细胞器等,使细胞能够在饥饿环境下得以生存,细胞自噬和神经退行性疾病、衰老以及肿瘤相关。有研究发现电离辐射如γ射线,非电离辐射如紫外线等均会诱导细胞自噬效应,本研究初步探索与人们日常生活密切相关的工频磁场(50Hz0.4mT)对中国仓鼠肺成纤维细胞(Chinese hamster lung cells, CHL)的细胞自噬的诱导。方法：CHL细胞培养24小时,更换新鲜的培养基后进行50Hz0.4mT工频磁场暴露30分钟、2小时、6小时、12小时或者24小时,磁场暴露后通过以下三种方法检测细胞自噬水平：透射电镜观察细胞内空泡变化,激光共聚焦显微镜检测细胞内GFP-LC3定位自噬小体情况以及Western Blot实验检测细胞内LC3-Ⅱ表达。结果：透射电子显微镜观察到,工频磁场暴露30分钟和24小时诱导细胞内空泡增加；激光共聚焦显微镜观察到,在GFP-LC3高表达的CHL细胞内,工频磁场暴露不同时间点诱导GFP-LC3亮点标记的自噬小体增加(P0.05),30分钟和24小时升高趋势较为明显；Western Blot技术检测发现磁场暴露促进自噬的特异性标记蛋白LC3-Ⅱ表达增加(P0.05),30分钟和24小时升高趋势较为明显；加入自噬小体与溶酶体融合的阻断剂氯喹后,磁场暴露30 min自噬流量增加(P0.05)。结论：这些研究提示本实验条件下50Hz0.4mT工频磁场暴露可能诱导CHL细胞自噬效应。
[Abstract]:Objective: with the development of electric power industry, the time and intensity of people contact with very low frequency magnetic field are increasing, and people pay more attention to the effect of very low frequency magnetic field. Early studies have shown that very low frequency magnetic fields can induce cell molecular changes, such as cell gap junction function (GJIC) decreased, cell membrane epidermal growth factor receptor (EGFR) and tumor necrosis factor receptor (TNFR) cluster, And gene and protein expression profile changes and so on. Autophagy is an important defense and protection mechanism. It degrades and reuses long-lived proteins, macromolecules and damaged organelles in order to make cells survive in hungry environment. Autophagy and neurodegenerative diseases, aging, and tumours are associated. Some studies have found that ionizing radiation such as 纬-rays and non-ionizing radiation such as ultraviolet radiation can induce autophagy effect. The purpose of this study was to explore the induction of autophagy of Chinese hamster lung fibroblasts (Chinese hamster lung cells, CHL) by power frequency magnetic field (50Hz0.4mT), which is closely related to people's daily life. Methods the cells were cultured for 24 hours. The fresh culture medium was replaced with 50Hz0.4mT power frequency magnetic field exposure for 30 minutes, 2 hours, 6 hours, 12 hours, or 24 hours, respectively. The level of autophagy was detected by three methods after magnetic field exposure: the changes of intracellular vacuoles were observed by transmission electron microscope (TEM), the intracellular GFP-LC3 localization autophagy was detected by laser confocal microscopy, and the expression of LC3- 鈪，
本文编号：2193288
[Abstract]:Objective: with the development of electric power industry, the time and intensity of people contact with very low frequency magnetic field are increasing, and people pay more attention to the effect of very low frequency magnetic field. Early studies have shown that very low frequency magnetic fields can induce cell molecular changes, such as cell gap junction function (GJIC) decreased, cell membrane epidermal growth factor receptor (EGFR) and tumor necrosis factor receptor (TNFR) cluster, And gene and protein expression profile changes and so on. Autophagy is an important defense and protection mechanism. It degrades and reuses long-lived proteins, macromolecules and damaged organelles in order to make cells survive in hungry environment. Autophagy and neurodegenerative diseases, aging, and tumours are associated. Some studies have found that ionizing radiation such as 纬-rays and non-ionizing radiation such as ultraviolet radiation can induce autophagy effect. The purpose of this study was to explore the induction of autophagy of Chinese hamster lung fibroblasts (Chinese hamster lung cells, CHL) by power frequency magnetic field (50Hz0.4mT), which is closely related to people's daily life. Methods the cells were cultured for 24 hours. The fresh culture medium was replaced with 50Hz0.4mT power frequency magnetic field exposure for 30 minutes, 2 hours, 6 hours, 12 hours, or 24 hours, respectively. The level of autophagy was detected by three methods after magnetic field exposure: the changes of intracellular vacuoles were observed by transmission electron microscope (TEM), the intracellular GFP-LC3 localization autophagy was detected by laser confocal microscopy, and the expression of LC3- 鈪，
本文编号：2193288
本文链接：https://www.wllwen.com/xiyixuelunwen/2193288.html
最近更新
教材专著
