低氧条件对小鼠ADSCs膜片形成及其生物学特性的影响
发布时间：2018-08-20 17:03
【摘要】：目的:观察低氧条件对小鼠脂肪间充质干细胞(ADSCs)膜片生物学性能的影响。方法:分离培养C57/BL小鼠ADSCs并用Vit C法构建细胞膜片;氯化钴(CoCl_2)模拟体内低氧环境,q PCR和Western Blot检测HIF-1α表达;组织学染色、透射电镜观察膜片形成;q PCR检测ADSCs干性及膜片分泌功能相关基因的表达。结果:成功构建ADSCs膜片;HIF-1α的表达随CoCl_2浓度的升高而升高;低氧条件下,形成细胞膜片较薄,但膜片内细胞连接更紧密;低氧环境可增强ADSCs干性,促使ADSCs膜片合成更多黏附分子、分泌更多保护性生长因子。结论:低氧条件更利于ADSCs膜片构建、存活和生物学功能维持。
[Abstract]:AIM: To observe the effects of hypoxia on the biological properties of adipose-derived mesenchymal stem cells (ADSCs) membrane in mice. Methods: ADSCs from C57/BL mice were isolated and cultured, and the cell membrane was constructed by Vit C method. Results: ADSCs membrane was successfully constructed, the expression of HIF-1a increased with the increase of CoCl_2 concentration, the formation of cell membrane was thinner under hypoxic condition, but the membrane cells were more closely connected; hypoxic environment could enhance the dryness of ADSCs, and promote the synthesis of more adhesion molecules and secretion of ADSCs membrane. CONCLUSION: Hypoxia is more conducive to the construction, survival and biological function of ADSCs.
【作者单位】： 军事口腔医学国家重点实验室 口腔疾病国家临床医学研究中心 陕西省口腔生物工程技术研究中心 第四军医大学口腔医院种植科;第四军医大学唐都医院血液科;
【基金】：国家自然科学基金(编号:81170984,81470775)
【分类号】：R329.2
，
本文编号：2194352
[Abstract]:AIM: To observe the effects of hypoxia on the biological properties of adipose-derived mesenchymal stem cells (ADSCs) membrane in mice. Methods: ADSCs from C57/BL mice were isolated and cultured, and the cell membrane was constructed by Vit C method. Results: ADSCs membrane was successfully constructed, the expression of HIF-1a increased with the increase of CoCl_2 concentration, the formation of cell membrane was thinner under hypoxic condition, but the membrane cells were more closely connected; hypoxic environment could enhance the dryness of ADSCs, and promote the synthesis of more adhesion molecules and secretion of ADSCs membrane. CONCLUSION: Hypoxia is more conducive to the construction, survival and biological function of ADSCs.
【作者单位】： 军事口腔医学国家重点实验室 口腔疾病国家临床医学研究中心 陕西省口腔生物工程技术研究中心 第四军医大学口腔医院种植科;第四军医大学唐都医院血液科;
【基金】：国家自然科学基金(编号:81170984,81470775)
【分类号】：R329.2
，
本文编号：2194352
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