胶囊渗透压泵控释rmIGFBPrP1对小鼠肝肾组织的影响及意义
发布时间：2018-08-21 07:18
【摘要】：目的： 胶囊渗透压泵植入野生型小鼠皮下并持续恒量释放重组鼠胰岛素样生长因子结合蛋白相关蛋白1 (rmIGFBPrP1),观察小鼠肝肾组织纤维化相关指标的表达情况,探讨rmIGFBPrP1对小鼠肝肾组织的影响及其可能的意义。 方法： 将26只雄性C57BL/6野生型小鼠按随机数字表法分为3组：正常对照组(8只),常规饮食持续4周；假手术组(8只),背部仅做一横向切口,缝合,常规饮食持续4周；模型组(10只),用1%水合氯醛麻醉小鼠,于小鼠前腿之间后背部行一0.5厘米左右横切口。用组织钳由切口处皮下向小鼠尾部方向撑出一个胶囊大小的空间,空间应大到胶囊泵体可以有一点移动,但不能让泵体轻易滑到小鼠侧翼去。行手术植入注满rmIGFBPrP1溶液的胶囊渗透压泵,输药管释出口要朝内塞入皮下,不可反向以致释出口刚好在伤口之下。胶囊泵连续恒定释放100μg/ml rmIGFBPrP1溶液,泵容积90μl,泵速0.11μl/h,持续4周。4周末处死各组小鼠,采集肝肾组织。HE染色和饱和苦味酸-天狼星红染色观察肝肾组织形态学改变及胶原沉积情况；免疫组织化学染色检测肝肾组织中IGFBPrP1、Smad3、p-Smad2/3和Ⅲ型胶原(CollagenⅢ)的表达和分布；原位末端转移酶标记法(TUNEL)检测肝细胞凋亡情况。 结果： 与对照组和假手术组相比,模型组肝组织中胶原纤维含量增多,IGFBPrP1、Smad3 p-Smad2/3、CollagenⅢ表达增强(P0.01)。模型组肾组织中仅IGFBPrP1表达较对照组和假手术组有所增强,胶原纤维和其它指标表达未见差异(P0.05)。TUNEL结果显示模型组肝细胞凋亡数目增多,与正常组和假手术组相比,差异有统计学意义(P0.01)。 结论： 1.外源性IGFBPrP1可促进小鼠肝细胞凋亡和肝纤维化的形成,而对小鼠肾组织影响甚微。 2. IGFBPrP1致小鼠脏器组织纤维化可能具有选择性。
[Abstract]:Objective: to investigate the expression of insulin like growth factor binding protein-associated protein 1 (rmIGFBPrP1) in mice by osmotic pump implantation into the subcutaneous of wild-type mice, and to observe the expression of insulin like growth factor binding protein-associated protein 1 (rmIGFBPrP1) in mice liver and kidney tissues. To investigate the effect of rmIGFBPrP1 on the liver and kidney of mice and its possible significance. Methods: 26 male C57BL/6 wild-type mice were randomly divided into three groups: normal control group (n = 8), sham operation group (n = 8) and sham operation group (n = 8). In the model group (n = 10), 1% chloral hydrate was used to anesthetize mice, and a 0.5 cm transverse incision was performed on the back of the front leg of the mice. Using tissue forceps from the incision subcutaneously to the tail of the mouse to open a capsule space, the space should be so large that the capsule pump body can move a little, but can not let the pump body slide to the mouse flank easily. The capsule osmotic pump filled with rmIGFBPrP1 solution was implanted surgically. The release outlet of the drug delivery tube should be inserted subcutaneously into the skin, so that the release outlet was just under the wound. The capsule pump continuously released 100 渭 g/ml rmIGFBPrP1 solution, pump volume 90 渭 l and pump velocity 0.11 渭 l / h. The mice were killed for 4 weeks at the end of 4 weeks. The liver and kidney tissues were stained with HE and saturated picric acid-Sirius red staining to observe the changes of liver and kidney morphology and collagen deposition. Immunohistochemical staining was used to detect the expression and distribution of IGFBPrP1Smad3 p-Smad2 / 3 and type 鈪，
本文编号：2194960
[Abstract]:Objective: to investigate the expression of insulin like growth factor binding protein-associated protein 1 (rmIGFBPrP1) in mice by osmotic pump implantation into the subcutaneous of wild-type mice, and to observe the expression of insulin like growth factor binding protein-associated protein 1 (rmIGFBPrP1) in mice liver and kidney tissues. To investigate the effect of rmIGFBPrP1 on the liver and kidney of mice and its possible significance. Methods: 26 male C57BL/6 wild-type mice were randomly divided into three groups: normal control group (n = 8), sham operation group (n = 8) and sham operation group (n = 8). In the model group (n = 10), 1% chloral hydrate was used to anesthetize mice, and a 0.5 cm transverse incision was performed on the back of the front leg of the mice. Using tissue forceps from the incision subcutaneously to the tail of the mouse to open a capsule space, the space should be so large that the capsule pump body can move a little, but can not let the pump body slide to the mouse flank easily. The capsule osmotic pump filled with rmIGFBPrP1 solution was implanted surgically. The release outlet of the drug delivery tube should be inserted subcutaneously into the skin, so that the release outlet was just under the wound. The capsule pump continuously released 100 渭 g/ml rmIGFBPrP1 solution, pump volume 90 渭 l and pump velocity 0.11 渭 l / h. The mice were killed for 4 weeks at the end of 4 weeks. The liver and kidney tissues were stained with HE and saturated picric acid-Sirius red staining to observe the changes of liver and kidney morphology and collagen deposition. Immunohistochemical staining was used to detect the expression and distribution of IGFBPrP1Smad3 p-Smad2 / 3 and type 鈪，
本文编号：2194960
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