慢性应激大鼠外周血促炎细胞因子和肠道微生态的变化

发布时间：2018-08-22 16:37

【摘要】：目的通过观察慢性应激大鼠外周血促炎细胞因子的表达和肠道微生态的变化,探讨慢性应激对于其体内炎症和肠道微生物的影响。方法对大鼠序贯采用束缚、强迫游泳和噪声三种刺激48 d,分别将进行应激18、33、48 d时存活的大鼠作为慢性应激1组(n=20)、慢性应激2组(n=20)和慢性应激3组(n=20);将未受实验应激原刺激的20只大鼠作为正常对照组。检测并比较4组大鼠一般状况、外周血的促炎细胞因子和肠道微生态状况。结果对照组大鼠的饮食、精神与活动状态良好。3个慢性应激组大鼠均呈现食欲不好、活动减少、毛发无光泽、精神萎靡和对外界刺激反应迟钝,大鼠上述症状、体征随应激时间的延长而明显,即按正常组、慢性应激1、2、3组之序,症状、体征依次加重。随应激程度的递增(正常组→慢性应激1组→2组→3组),大鼠血清白细胞介素(IL)-1β、IL-6、肿瘤坏死因子(TNF)-α和干扰素(INF)-γmRNA相对表达量递升(P均0.01);大鼠盲肠的肠杆菌和肠球菌定量[集落形成单位(CFU/g)]递升(P均0.01),乳杆菌和双歧杆菌定量则递降(P均0.01)。结论慢性应激可显著增加大鼠外周血促炎细胞因子水平和改变肠道微生态,变化程度与应激程度相关。降低促炎细胞因子水平和/或改善肠道微生态状态可能有利于改善其慢性应激状态。
[Abstract]:Objective to investigate the effects of chronic stress on inflammation and intestinal microorganism in rats with chronic stress by observing the expression of pro-inflammatory cytokines in peripheral blood and the changes of intestinal microecology. Methods Sequential restraint was used in rats. After 48 days of forced swimming and noise stimulation, the rats survived at 1833 days as chronic stress group 1 (n = 20), chronic stress group 2 (n = 20) and chronic stress group 3 (n = 20), and 20 rats who were not stimulated by experimental stressors as normal control group (n = 20), the rats were divided into three groups: chronic stress group 1 (n = 20), chronic stress group 2 (n = 20) and chronic stress group 3 (n = 20). The general status, proinflammatory cytokines and intestinal microecology in peripheral blood of 4 groups were detected and compared. Results the diet, mental and activity state of the rats in the control group were good. The rats in the three chronic stress groups showed poor appetite, decreased activity, dull hair, low spirit and slow response to external stimuli. The signs were obvious with the prolongation of stress time, that is, according to the order of normal group and chronic stress group, the symptoms and signs were aggravated in turn. With the increase of stress degree (normal group, chronic stress group 1 and group 2, group 3), the relative expression of interleukin-1 (IL)-1 尾 -IL-6, tumor necrosis factor (TNF)-伪 and interferon (INF)-纬 mRNA in serum of rats increased (P0.01); Enterobacter and enterococci in caecum of rats increased (P0.01). Quantitative [colony forming unit (CFU/g)] was increased (P0.01), while Lactobacillus and Bifidobacterium decreased (P0.01). Conclusion chronic stress can significantly increase the level of proinflammatory cytokines in peripheral blood of rats and change the intestinal microecology, and the degree of change is related to the degree of stress. Lowering the level of proinflammatory cytokines and / or improving the intestinal microecological state may be beneficial to improve their chronic stress state.
【作者单位】：四川省人民医院肿瘤科;中航工业·成飞医院;四川大学华西医院肿瘤科;
【基金】：国家自然科学基金(81402402)
【分类号】：R36

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