婴儿粪便长双歧杆菌的分离与多样性分析

发布时间：2018-08-23 19:17

【摘要】：本研究旨在分析婴儿粪便长双歧杆菌的多样性,采用改良MRS培养基从哈尔滨地区健康婴儿粪便中分离双歧杆菌,采用生理生化实验结合16S rDNA和热应激蛋白60基因(heat-shock protein 60,hsp60)同源性分析鉴定分离株,利用同源性分析及随机扩增多态性DNA(random amplified polymorphism DNA,RAPD)、多位点序列分型(multilocus sequence typing,MLST)技术进一步分析长双歧杆菌多态性。实验从11个婴儿体内分离得到18株厌氧的细菌菌株,经形态学分析、生理生化实验、16S rDNA测序及hsp60测序实验发现,其中7株为长双歧杆菌长亚种,3株为长双歧杆菌婴儿亚种。RAPD和MLST结果表明:7株长双歧杆菌长亚种为6个基因型,3株长双歧杆菌婴儿亚种为2个基因型,上述结果说明不同人源婴儿粪便长双歧杆菌基因型差异较大。
[Abstract]:The aim of this study was to analyze the diversity of bifidobacterium in infant faeces and to isolate Bifidobacterium from healthy infant faeces in Harbin area by using modified MRS medium. The isolates were identified by physiological and biochemical experiments combined with 16s rDNA and heat stress protein 60 gene (heat-shock protein 60hsp60) homology analysis. The polymorphism of Bifidobacterium longii was further analyzed by homology analysis, random amplified polymorphic DNA (random amplified polymorphism DNA,RAPD and multilocus sequence typing (multilocus sequence typing,MLST). Eighteen anaerobic bacterial strains were isolated from 11 infants. The results of morphological analysis, physiological and biochemical experiments, 16s rDNA sequencing and hsp60 sequencing showed that, The results of RAPD and MLST showed that 7 strains of bifidobacterium were 6 genotypes and 3 strains of bifidobacterium long subspecies were 2 genotypes of Bifidobacterium longifera, 3 strains of bifidobacterium longitudinalis were subspecies of Bifidobacterium longifera, and 3 strains of bifidobacteria were subspecies of bifidobacteria. The above results indicated that the genotypes of Bifidobacterium longus in feces of different human infants were different.
【作者单位】：东北农业大学乳品科学教育部重点实验室;
【基金】：国家自然科学基金面上项目(31671917)
【分类号】：R375

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