胰岛素抵抗小鼠血清微小RNA的芯片及信息学分析

发布时间：2018-08-23 20:21

【摘要】：目的:探讨胰岛素抵抗小鼠血清水平微小RNA(microRNAs)变化图谱及相关microRNAs的可能作用机制。方法:以高脂饲料喂养昆明小鼠,制备胰岛素抵抗模型;以微阵列芯片技术分析胰岛素抵抗小鼠及正常小鼠血清水平microRNAs变化图谱并以实时荧光定量PCR进行验证;以miRanda数据库对差异microRNAs进行靶点基因预测;以miRBase数据库获得与胰岛素抵抗相关的microRNAs序列;基于microRNAs序列,应用STRING在线分析工具(http://string.embl.de/)预测蛋白质相互作用关系。结果:胰岛素抵抗小鼠血清中miR-125、miR-126、miR-143、miR-30a、miR-199a、miR-127、miR-184、miR-30e、miR-134、miR-195、miR-206、miR-429、miR-212、miR-362、miR-382、miR-154和miR-466h表达显著上调,miR-211、miR-504、miR-877和miR-1930显著下调。与胰岛素抵抗密切相关的miR-143可以结合脂肪量及肥胖相关蛋白(FTO)的3’-UTR,并且FTO与Rpgrip1l、Tmem18、Mc4r、Npy、Hhex、Tcf712、Cdkal1、Slc30a8、Igf2bp2和Thada相互作用。结论:在高脂饮食诱导的胰岛素抵抗小鼠血清中有21种microRNAs相比正常小鼠出现了显著变化,表达显著上调的有17个。应用在线分析工具发现与胰岛素抵抗密切相关的miR-143可以调节FTO蛋白表达,并且FTO与另外10种与糖尿病发生发展相关的蛋白相互作用,这将有助于对胰岛素抵抗机制的全面了解。
[Abstract]:Aim: to investigate the microRNA (microRNAs) profile of insulin resistant mice and the possible mechanism of microRNAs. Methods: Kunming mice were fed with high fat diet to make insulin resistance model, and microarray technique was used to analyze the changes of serum microRNAs in mice with insulin resistance and normal mice, and the results were verified by real-time fluorescence quantitative PCR. The target gene of differential microRNAs was predicted by miRanda database, the microRNAs sequence related to insulin resistance was obtained by miRBase database, and the protein interaction was predicted by STRING online analysis tool (http://string.embl.de/) based on microRNAs sequence. Results: the expression of miR-125,miR-126,miR-143,miR-30a,miR-199a,miR-127,miR-184,miR-30e,miR-134,miR-195,miR-206,miR-429,miR-212,miR-362,miR-382,miR-154 and miR-466h in serum of insulin-resistant mice was significantly up-regulated, and the expression of miR-504 miR-877 and miR-1930 were down-regulated significantly. MiR-143, which is closely related to insulin resistance, can bind to fat content and 3H-UTR-UTR-UTRof the obesity-associated protein (FTO), and FTO interacts with Rpgrip1l,Tmem18,Mc4r,Npy,Hhex,Tcf712,Cdkal1,Slc30a8,Igf2bp2 and Thada. Conclusion: there are 21 kinds of microRNAs in the serum of insulin resistance mice induced by high fat diet, and 17 of them are up-regulated. It is found that miR-143, which is closely related to insulin resistance, can regulate the expression of FTO protein, and that FTO interacts with 10 other proteins related to the development of diabetes mellitus, which will contribute to a comprehensive understanding of the mechanism of insulin resistance.
【作者单位】：河北北方学院医学检验学院生化教研室;
【基金】：张家口市科技局项目(No.12110065G-5) 河北省教育厅项目(No.QN2016175)
【分类号】：R363

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