抗B7-H3单克隆抗体抑制雨蛙肽诱导的小鼠急性胰腺炎炎症反应和组织损伤
发布时间：2018-08-24 11:12
【摘要】：目的探究抗B7-H3单克隆抗体(m Ab)在雨蛙肽诱导的急性胰腺炎(AP)中的作用。方法将小鼠随机分为对照组、AP组、抗B7-H3 m Ab治疗组。采用腹腔注射雨蛙肽的方法制备小鼠AP模型,治疗组于造模前1 h皮下注射抗B7-H3 m Ab,于造模后6、12、24 h,收集各组小鼠血液、胰腺及肺组织。采用Western blot法和免疫组织化学染色检测对照组及AP组小鼠胰腺组织B7-H3蛋白表达。应用全自动生化免疫分析仪检测各组小鼠血清淀粉酶及脂肪酶活性;胰腺组织湿干比评估各组小鼠胰腺组织水肿变化;HE染色评估各组小鼠胰腺及肺组织病理变化,利用ELISA检测各组小鼠血清中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、IL-1β水平。结果 B7-H3蛋白在AP模型小鼠胰腺组织高表达,且在12 h左右达到高峰;AP组血清淀粉酶及脂肪酶明显高于对照组,抗B7-H3 m Ab处理后明显降低;AP组胰腺及肺组织出现明显炎症反应,治疗组炎症反应明显减弱,胰腺组织湿干比大幅降低;AP组TNF-α、IL-6、IL-1β水平随时间延长而升高,12 h达到高峰后开始下降,而治疗组促炎因子水平相对降低。结论 B7-H3在雨蛙肽诱导的AP模型中存在高表达,抗B7-H3 m Ab可以减弱炎症反应,缓解胰腺及肺组织的损伤。
[Abstract]:Objective to investigate the role of anti-B7-H3 monoclonal antibody (m Ab) in acute pancreatitis (AP) induced by rainfrog peptide. Methods mice were randomly divided into control group (AP group) and anti B7-H3 m Ab group. The AP model of mice was established by intraperitoneal injection of rain frog peptide. In the treatment group, anti B7-H3 m Ab, was injected subcutaneously 1 hour before the model was made. The blood, pancreas and lung tissues of each group were collected. The expression of B7-H3 protein in pancreas of control group and AP group was detected by Western blot and immunohistochemical staining. The activities of serum amylase and lipase in each group were detected by automatic biochemical immunoassay, the changes of pancreatic edema in each group were evaluated by wet / dry ratio of pancreatic tissue and the pathological changes of pancreas and lung in each group were evaluated by HE staining. Serum levels of tumor necrosis factor 伪 (TNF- 伪) and interleukin-6 (IL-6) IL-1 尾 were measured by ELISA. Results the expression of B7-H3 protein in pancreatic tissue of AP model mice was high, and the levels of serum amylase and lipase in AP group were significantly higher than those in control group at about 12 h, and the inflammatory reaction in pancreas and lung tissue of AP group was obviously decreased after treatment with anti-B7-H3 m Ab. In the treatment group, the inflammatory response was obviously weakened, the wet / dry ratio of pancreatic tissue was significantly decreased, and the level of TNF- 伪, IL-6 and IL-1 尾 in the AP group increased with the prolongation of time and reached its peak at 12 h, but the level of proinflammatory factor decreased relatively in the treatment group. Conclusion there is a high expression of B7-H3 in the AP model induced by rain frog peptide. Anti B7-H3 m Ab can attenuate the inflammatory reaction and alleviate the injury of pancreas and lung tissue.
【作者单位】： 苏州大学附属第一医院消化内科;
【基金】：江苏省自然科学基金(BK20131157)
【分类号】：R392
本文编号：2200644
[Abstract]:Objective to investigate the role of anti-B7-H3 monoclonal antibody (m Ab) in acute pancreatitis (AP) induced by rainfrog peptide. Methods mice were randomly divided into control group (AP group) and anti B7-H3 m Ab group. The AP model of mice was established by intraperitoneal injection of rain frog peptide. In the treatment group, anti B7-H3 m Ab, was injected subcutaneously 1 hour before the model was made. The blood, pancreas and lung tissues of each group were collected. The expression of B7-H3 protein in pancreas of control group and AP group was detected by Western blot and immunohistochemical staining. The activities of serum amylase and lipase in each group were detected by automatic biochemical immunoassay, the changes of pancreatic edema in each group were evaluated by wet / dry ratio of pancreatic tissue and the pathological changes of pancreas and lung in each group were evaluated by HE staining. Serum levels of tumor necrosis factor 伪 (TNF- 伪) and interleukin-6 (IL-6) IL-1 尾 were measured by ELISA. Results the expression of B7-H3 protein in pancreatic tissue of AP model mice was high, and the levels of serum amylase and lipase in AP group were significantly higher than those in control group at about 12 h, and the inflammatory reaction in pancreas and lung tissue of AP group was obviously decreased after treatment with anti-B7-H3 m Ab. In the treatment group, the inflammatory response was obviously weakened, the wet / dry ratio of pancreatic tissue was significantly decreased, and the level of TNF- 伪, IL-6 and IL-1 尾 in the AP group increased with the prolongation of time and reached its peak at 12 h, but the level of proinflammatory factor decreased relatively in the treatment group. Conclusion there is a high expression of B7-H3 in the AP model induced by rain frog peptide. Anti B7-H3 m Ab can attenuate the inflammatory reaction and alleviate the injury of pancreas and lung tissue.
【作者单位】： 苏州大学附属第一医院消化内科;
【基金】：江苏省自然科学基金(BK20131157)
【分类号】：R392
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