血清淀粉样蛋白A诱导中性粒细胞胞外诱捕网形成

发布时间：2018-08-24 14:56

【摘要】：目的探究血清淀粉样蛋白A(SAA)能否在体外诱导中性粒细胞形成中性粒细胞胞外诱捕网(NETs)。方法建立稳定的体外诱导NETs形成方法,包括外周血中性粒细胞的分离、细胞体外培养、NETs的诱导和形态学观察。提取健康志愿者的外周血中性粒细胞,分为阴性对照(NC)组、SAA组和脂多糖(LPS)组,给予相应的刺激后,观察NETs的形成并计算百分率,酶联免疫吸附试验(ELISA)检测培养上清中人组蛋白3(h3)的含量。结果提取的外周血中性粒细胞纯度与细胞活力均在95%以上。显微镜下观察到部分细胞的细胞核失去原有核型,向外扩散形成网状,即NETs。与NC组相比,接受SAA刺激的中性粒细胞有更多的NETs形成(P0.05)。培养液上清中的h3含量显著高于NC组(P0.05)。结论 SAA可在体外诱导中性粒细胞形成NETs。
[Abstract]:Objective to investigate whether serum amyloid A (SAA) can induce neutrophils to form neutrophil extracellular entrapment net (NETs).) in vitro. Methods A stable method was established to induce the formation of NETs in vitro, including the isolation of peripheral blood neutrophils, the induction of cell culture in vitro and the morphological observation. The peripheral blood neutrophils of healthy volunteers were divided into negative control (NC) group and lipopolysaccharide (LPS) group. After stimulation, the formation of NETs was observed and the percentage of NETs was calculated. The content of human histone 3 (H3) in culture supernatant was detected by (ELISA). Results the purity and viability of neutrophils were above 95%. Microscopically, the nuclei of some cells lost their original karyotype and diffused outward to form a reticular form, that is, NETs.. Compared with NC group, neutrophils stimulated by SAA had more NETs formation (P0.05). The content of H3 in supernatant of culture medium was significantly higher than that in NC group (P0.05). Conclusion SAA can induce neutrophils to form NETs. in vitro.
【作者单位】：天津医科大学医学检验学院免疫学教研室;天津市天津医院;天津医科大学总医院;
【基金】：天津市应用基础与前沿技术研究计划项目(14JCYBJC25600)
【分类号】：R392

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