帕金森病模型小鼠脾悬液中辅助性T细胞17和髓系抑制性细胞数量的变化及其意义

发布时间：2018-08-25 07:45

【摘要】：目的探讨帕金森病(PD)模型小鼠脾悬液中辅助性T细胞17(Th17)和髓系抑制性细胞(MDSC)数量的变化及其意义。方法将40只C57BL/6小鼠随机分为PD组(A组)、PD+吉西他滨组(B组)、吉西他滨组(C组)与对照组(D组),每组10只小鼠。A组和B组予1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)(30 mg/kg)连续腹腔注射7 d;B组和C组于第1 d,7 d腹腔注射吉西他滨(120 mg/kg);D组予等体积生理盐水腹腔注射。第8 d进行行为学实验后,检测各组小鼠黑质区酪氨酸羟化酶(TH)阳性细胞数量和脾悬液Th17和MDSC细胞百分率。结果与建模前比较,A组、B组小鼠调头时间、爬杆时间明显延长,悬挂时间明显缩短(均P0.05)。与C组、D组比较,建模后A组、B组小鼠调头时间、爬杆时间明显延长,悬挂时间明显缩短(均P0.05)。A组、B组小鼠中脑黑质区TH阳性细胞数量较C组和D组明显减少(均P0.05)。A组、B组小鼠脾悬液中Th17、MDSC细胞百分数较C组和D组明显升高(均P0.05);且B组小鼠脾悬液中Th17细胞百分数明显高于A组(P0.05),A组小鼠脾悬液中MDSC细胞百分数明显高于B组(P0.05)。Pearson相关性分析结果显示,A组和B组小鼠Th17和MDSC细胞百分数间均无相关性(r=-0.319,r=0.04,均P0.05)。结论 PD模型小鼠Th17、MDSC数量增加,可能参与了PD的发生发展。MDSC有可能发挥了抑制Th17扩增的免疫调节作用。
[Abstract]:Objective to investigate the changes and significance of helper T cell 17 (Th17) and myeloid inhibitory cell (MDSC) (MDSC) in spleen suspension of (PD) mice with Parkinson's disease. Methods 40 C57BL/6 mice were randomly divided into PD group (group A), gemcitabine group (group B), gemcitabine group (group C) and control group (group D). Group B and group C received intraperitoneal injection of gemcitabine (120 mg/kg) intraperitoneally on the 7th day after 7 days of irradiation with normal saline of the same volume. The number of tyrosine hydroxylase (TH) positive cells in substantia nigra and the percentage of Th17 and MDSC cells in spleen suspension were measured after behavioral experiment on the 8th day. Results compared with those before modeling, the turning time, climbing time and suspending time of group A and B mice were significantly prolonged (P0.05). Compared with group C and group D, the time of turning head and climbing pole in group A and group B were significantly prolonged after modeling. The suspension time was significantly shortened (P0.05). The number of TH positive cells in the substantia nigra of group A was significantly lower than that in group C and group D (P0.05). The percentage of Th17,MDSC cells in splenic suspension of group A was significantly higher than that of group C and group D (P0.05). The percentage of Th17 cells in splenic suspension of group B was significantly higher than that in group A (P0.05). Pearson correlation analysis showed that there was no correlation between the percentage of Th17 and MDSC in group A and group B (r-0.319r0.04, P0.05). Conclusion the increased number of Th17,MDSC in PD model mice may be involved in the development of PD. MDSC may play an immunoregulatory role in inhibiting Th17 amplification.
【作者单位】：江苏大学附属医院神经内科;江苏大学附属医院中心实验室;
【基金】：江苏省自然科学基金(BK20160548) 江苏省“333工程”科研项目(BRA2014173) 江苏省“六大人才高峰”科研项目(WSN038) 镇江市重点研发计划(SH2016032) 镇江市社会发展科技计划项目(SH2015047)
【分类号】：R-332;R742.5

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