高碘对氧化损伤的影响

发布时间：2018-08-27 19:30

【摘要】：目的：本此实验通过给大鼠喂高碘水,建立高碘大鼠模型,通过检测同型半胱氨酸(HCY)、丙二醛(MDA)、抗氧化指标(超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)指标,观察不同浓度的高碘对氧化损伤的影响,并探讨氧化损伤的机制。方法： 1.实验动物与分组：将32只断乳1个月左右远交封闭群的SPF／VAF级wistar大鼠随机分为4组,分别为正常对照(NC,水碘含量为5μg／L)组和10倍碘(10HI,2000μg／L)组、50倍碘(50HI,10000μg／L)组、100倍碘(100HI,20000μg／L)组,每组8只,雌雄各半。 2.记录大鼠体重数据：采用自由饮水方式进行染毒,连续喂养6个月。 3.测定指标：染毒结束后,测定血清同型半胱氨酸、丙二醛、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)含量。数据采用SPSS16.0进行统计学分析。 [结果] 1.大鼠体重的检测结果说明,无论雄性还是雌性,不同高碘组的大鼠体重与NC组大鼠对照均略有下降,但这个差异均没有统计学意义。并且不同性别的大鼠体重之间对照,这个差异也没有统计学意义。 2.大鼠血清中,HCY和MDA含量的检测结果说明：与NC组对照,无论雄性还是雌性,50、100HI组的大鼠血清中HCY和MDA含量均较高,差异有统计学意义(P0.05或P0.01)。而不同性别大鼠血清中HCY和MDA含量间比较,差异无统计学意义。 3.大鼠血清中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)测定结果表明,50、100HI组大鼠血清中SOD和GSH-Px含量均降低,差异有统计学意义(P0.05或P0.01)。而不同性别大鼠血清中HCY和MDA含量间比较,差异无统计学意义。 [结论] 1.无论是雄性还是雌性,不同高碘组的大鼠体重与NC组大鼠相比较均略有下降,但这差异都没有统计学意义。并且不同性别的大鼠体重间比较,这差异也没有统计学意义。 2.10倍高碘对大鼠血清中CY、MDA、SOD、GSH-Px未有影响,而50、100倍高碘可引起大鼠血清中HCY及MDA含量上升,SOD、GSH-Px含量下降。提示高碘可导致发生氧化反应增强,抗氧化减弱,血管内皮收到损伤。 3.不同性别大鼠血清中HCY、MDA、SOD、GSH-Px含量间比较,差异无统计学意义。
[Abstract]:Objective: to establish a rat model of hyperiodized rat by feeding high iodine water, and to detect the (SOD), glutathione peroxidase (GSH-Px) index of homocysteine (HCY), malondialdehyde (MDA),). The effects of different concentrations of iodine on oxidative damage were observed and the mechanism of oxidative damage was discussed. Methods: 1. Experimental animals and groups: 32 SPF/VAF wistar rats were randomly divided into 4 groups. They were normal control (5 渭 g / L in NC, water) and 10 times iodine (10 ~ 2 000 渭 g / L) groups with 50 times iodine (10 ~ 10 000 渭 g / L) and 100 ~ 100 times iodine (~ 20 000 渭 g / L) in each group, 8 rats in each group, female and male were half of each group. The rats' body weight data were recorded: exposed to free drinking water and fed continuously for 6 months. The content of homocysteine malondialdehyde (MDA) superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured. The data were analyzed by SPSS16.0. [result] 1. The results showed that the weight of rats in different iodine groups was slightly lower than that in NC group, but the difference was not statistically significant. And there was no significant difference in body weight between different genders. 2. 2. The results showed that the content of HCY and MDA in serum of male and female rats was higher than that of NC group (P0.05 or P0.01). However, there was no significant difference in serum HCY and MDA levels between male and female rats. The results of serum superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) assay showed that the contents of SOD and GSH-Px in the serum of rats treated with X50100HI decreased significantly (P0.05 or P0.01). However, there was no significant difference in serum HCY and MDA levels between different sex rats. [conclusion] 1. No matter male or female, the weight of rats in different iodine groups was slightly lower than that in NC group, but the difference was not statistically significant. There was no significant difference in body weight between rats of different genders. 2.10 times excess iodine had no effect on serum CY,MDA,SOD,GSH-Px, while 50100 times iodine could increase the contents of HCY and MDA in serum and decrease the content of GSH-Px in serum of rats. These results suggest that excessive iodine can lead to increased oxidation reaction, decreased oxidation resistance and injury of vascular endothelium. There was no significant difference in serum HCY,MDA,SOD,GSH-Px content between different sex rats.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363

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