TLR4-NOD2协同信号传递增强树突状细胞抗结核分枝杆菌感染的作用研究
发布时间：2018-08-30 10:20
【摘要】：目的研究TRL4-NOD2(T4N2)信号传递增强树突状细胞(dendritic cell,DC)抗结核分枝杆菌(MTB)感染机制,为结核病(tuberculosis,TB)的免疫防治提供参考。方法分别用TLR4配体LPS、NOD2配体MDP和T4N2双配体刺激DC后,ELISA法定量检测1h、6h、12h、24h和48h上清液中IL-6和IL-12。DC经MTB感染再用LPS、MDP以及T4N2双配体刺激,24h后分别收集细胞培养并计数细胞内生长的菌落数,判断细菌生长的抑制率。结果ELISA显示T4N2双信号刺激DC后,细胞因子IL-6在1h、6h、12h、24h和48h浓度分别为(86.4±0.34)pg/ml、(92.2±0.69)pg/ml、(93.6±0.69)pg/ml、(96.0±1.73)pg/ml和(107.8±4.53)pg/ml;IL-12在1h、6h、12h、24h和48h浓度分别为(94.6±4.07)pg/ml、(100.9±4.45)pg/ml、(97.9±2.96)pg/ml、(112.4±9.28)pg/ml和(132.8±1.49)pg/ml。不同时间段T4N2双信号刺激较LPS、MDP刺激显著增多(P0.05);T4N2双信号活化的DC对MTB生长抑制率显著增强。结论 T4N2信号传递能协同增强DC的活化,活化的DC可抑制MTB的生长。
[Abstract]:Objective to study the mechanism of TRL4-NOD2 (T4N2) signal transduction enhanced dendritic cells (dendritic cell,DC) against (MTB) infection of Mycobacterium tuberculosis and to provide a reference for the prevention and treatment of tuberculosis (tuberculosis,TB). Methods TLR4 ligand LPS,NOD2 ligand MDP and T4N2 double ligand stimulated DC were used to detect the number of cells cultured in the supernatant of IL-6 and IL-12.DC after MTB infection and LPS,MDP and T4N2 double ligand stimulation for 24 h and 48 h, respectively. To judge the inhibition rate of bacteria growth. 缁撴灉ELISA鏄剧ずT4N2鍙屼俊鍙峰埡婵，
本文编号：2212778
[Abstract]:Objective to study the mechanism of TRL4-NOD2 (T4N2) signal transduction enhanced dendritic cells (dendritic cell,DC) against (MTB) infection of Mycobacterium tuberculosis and to provide a reference for the prevention and treatment of tuberculosis (tuberculosis,TB). Methods TLR4 ligand LPS,NOD2 ligand MDP and T4N2 double ligand stimulated DC were used to detect the number of cells cultured in the supernatant of IL-6 and IL-12.DC after MTB infection and LPS,MDP and T4N2 double ligand stimulation for 24 h and 48 h, respectively. To judge the inhibition rate of bacteria growth. 缁撴灉ELISA鏄剧ずT4N2鍙屼俊鍙峰埡婵，
本文编号：2212778
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