蝇蛆肽对衰老小鼠的作用及机制的初探

发布时间：2018-08-30 15:05

【摘要】：目的:探讨蝇蛆肽对衰老小鼠的作用及其机制。方法:将60只小鼠随机分为6组,除空白组外,其余五组用D-半乳糖致衰老,分为衰老模型组、维生素C组以及蝇蛆肽低、中、高剂量组(2、4、8 g/kg)。测定小鼠的脾脏和胸腺指数。采用羟胺法测定小鼠肝脏和脑及血清中的超氧化物歧化酶(SOD)活力,可见光法测定过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)的活力,以硫代巴比妥酸(TBA)法测定丙二醛(MDA)的含量。结果:与空白组相比,模型组小鼠脾指数、胸腺指数及肝脏指数均有显著降低(p0.05),蝇蛆肽低、中、高剂量组脏器指数显著增高(p0.05)。与空白组比较,模型组肝、脑中SOD、CAT、GSH-Px均降低,且MDA含量增高(p0.05),血清中SOD、CAT降低、MDA含量增高(p0.05);与模型组比较,蝇蛆肽低、中、高剂量组肝、脑组织中SOD、CAT、GSH-Px均显著增高、MDA含量显著降低(p0.05),血清中SOD、CAT显著增高、MDA含量显著降低(p0.05)。结论:蝇蛆肽通过改变衰老小鼠的脏器指数而对其产生免疫调节作用,可显著提高小鼠肝脏和脑组织中的SOD、CAT、GSH-Px的活力,降低MDA的含量(p0.05),从而改变机体抗氧化能力。蝇蛆肽对衰老小鼠的影响机制可能与增强其体内抗氧化酶活力及改变脏器指数相关。
[Abstract]:Objective: to investigate the effect and mechanism of fly maggot peptide on aging mice. Methods: sixty mice were randomly divided into 6 groups. Except the blank group, the other five groups were divided into aging model group, vitamin C group, low, medium and high dose group (2: 48 g/kg) with D-galactose. Spleen and thymus index of mice were measured. The activity of superoxide dismutase (SOD) in liver, brain and serum of mice was determined by hydroxylamine method, the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) were measured by visible light method, and the content of malondialdehyde (MDA) was determined by thiobarbituric acid (TBA) method. Results: compared with the blank group, the spleen index, thymus index and liver index of the model group were significantly decreased (p0.05), and the organ index of the middle and high dose groups were significantly higher than that of the control group (p0.05). Compared with the blank group, the content of SOD,CAT,GSH-Px in liver and brain of model group was decreased, and the content of MDA was increased (p0.05), and the content of SOD,CAT in serum was decreased (p0.05), compared with the model group, the content of maggot peptide was lower in model group, lower in middle dose group, and higher in high dose group. The content of SOD,CAT,GSH-Px in brain tissue was significantly higher than that in control group (p0. 05). The content of SOD,CAT in serum was significantly higher than that in control group (p0. 05). Conclusion: maggot peptide can significantly increase the activity of SOD,CAT,GSH-Px in liver and brain and decrease the content of MDA by changing the viscera index of aging mice (p0.05), which can change the antioxidant ability of the body. The mechanism of the effect of fly maggot peptide on aging mice may be related to the enhancement of antioxidant enzyme activity and the change of organ index.
【作者单位】：贵州医科大学;
【基金】：贵州省2014年大学生创新创业训练计划项目(201410660019) 教育部2016年高校大学生创新创业训练计划项目(201610660310)
【分类号】：R339.38

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