基因分型诊断在肠道病毒性中枢神经系统感染中的应用探讨
发布时间：2018-08-31 15:10
【摘要】：目的研究基因分型诊断在肠道病毒性中枢神经系统感染病原学诊断的临床应用价值。 方法选取我院2006-06-2009-08期间诊断为病毒性脑炎的住院患儿共50例,均于入院的当天抽取脑脊液。试验先经种属特异性引物扩增,筛选出肠道病毒样本,然后分别用测序VP1及VP2序列的方法进行分型。试验同时设立阳性对照及阴性对照。 结果50例临床样本中,共有45例临床样本检测出EV RNA。其中,经VP1序列分型方法可成功扩增分型38例,反应产物为357 bp的片段。经VP2序列分型方法可成功扩增分型34例,扩增产物为584 bp的片段。经BLAST比对结果显示：两者在分型结果上有较高的一致性及互补性。 结论①经VP1序列分型方法可以成功检测临床常见肠道病毒中的大多数血清型。②联合VP2序列分型方法较单用VP1序列分型方法,分型率及敏感性更高,值得临床上进一步推广。
[Abstract]:Objective to study the clinical value of genotyping in etiological diagnosis of enterovirus central nervous system infection. Methods 50 hospitalized children with viral encephalitis diagnosed in our hospital from June 2006 to August 2009-08 were selected and cerebrospinal fluid (CSF) was collected on the day of admission. The samples of enterovirus were screened by species-specific primers, and then the VP1 and VP2 sequences were sequenced. Both positive and negative controls were established in the experiment. Results EV RNA. was detected in 45 of 50 clinical samples. Among them, 38 cases were successfully amplified by VP1 sequence typing, and the reaction product was 357 bp fragment. 34 cases were successfully amplified by VP2 sequence typing, and the amplified product was 584 bp. The results of BLAST comparison showed that there was a high consistency and complementarity in the typing results between the two groups. Conclusion (1) the VP1 sequence typing method can successfully detect most serotypes of enterovirus combined with VP2 sequence typing method, and the typing rate and sensitivity are higher than that of single VP1 sequence typing method, which is worthy of further clinical promotion.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R373.2
[Abstract]:Objective to study the clinical value of genotyping in etiological diagnosis of enterovirus central nervous system infection. Methods 50 hospitalized children with viral encephalitis diagnosed in our hospital from June 2006 to August 2009-08 were selected and cerebrospinal fluid (CSF) was collected on the day of admission. The samples of enterovirus were screened by species-specific primers, and then the VP1 and VP2 sequences were sequenced. Both positive and negative controls were established in the experiment. Results EV RNA. was detected in 45 of 50 clinical samples. Among them, 38 cases were successfully amplified by VP1 sequence typing, and the reaction product was 357 bp fragment. 34 cases were successfully amplified by VP2 sequence typing, and the amplified product was 584 bp. The results of BLAST comparison showed that there was a high consistency and complementarity in the typing results between the two groups. Conclusion (1) the VP1 sequence typing method can successfully detect most serotypes of enterovirus combined with VP2 sequence typing method, and the typing rate and sensitivity are higher than that of single VP1 sequence typing method, which is worthy of further clinical promotion.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R373.2
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