培元解郁方对几种抑郁模型小鼠的抗抑郁作用及机制研究

发布时间：2018-09-02 05:30

【摘要】：目的研究培元解郁方的抗抑郁作用和作用机制。方法采用利血平2.5 mg/kg腹腔注射,建立小鼠利血平抑郁模型;采用强迫游泳方法,建立急性应激小鼠抑郁模型;采用长期(14 d)束缚加噪声,建立慢性应激小鼠抑郁模型。观察培元解郁方对利血平抑郁模型小鼠的体温、眼睑下垂、出圈率的影响;对急性应激模型、慢性应激模型小鼠的强迫游泳不动时间的影响。采用酶联免疫吸附(ELISA)法检测急性应激模型小鼠脑中5-羟色胺(5-HT)、去甲肾上腺素(NA)及吲哚胺-2,3-双加氧酶(IDO)的含量;慢性应激模型小鼠脑中5-HT、IDO的含量。并采用荧光定量PCR检测慢性应急模型小鼠脑中IDO mRNA的表达情况。结果培元解郁方各剂量组均能拮抗利血平抑郁模型小鼠的体温下降(P0.01),此外高剂量还可改善利血平引起的眼睑下垂、运动不能的状况(P0.05),中剂量组可改善运动不能的状况(P0.05)。培元解郁方中、低剂量组均可缩短急性应激模型小鼠不动时间(P0.05、P0.01),且显著增加脑中5-HT、NA的含量(P0.05、P0.01)。培元解郁方高、中、低剂量组均可不同程度地缩短慢性应激模型小鼠强迫游泳不动时间、增加脑组织中5-HT的含量、降低IDO的含量并上调IDO mRNA的表达(P0.05)。结论培元解郁方对利血平、强迫游泳、慢性应激造成的小鼠抑郁症状均有一定的改善作用。其机制可能与增加抑郁模型小鼠脑中单胺类神经递质5-HT、NA含量,抑制IDO活性、下调IDO mRNA表达有关。
[Abstract]:Objective to study the antidepressant effect and mechanism of Peiyuan Jieyu prescription. Methods mice model of depression was established by intraperitoneal injection of reserpine 2.5 mg/kg, depression model of mice under acute stress was established by forced swimming, depression model of mice under chronic stress was established by long-term (14 d) restraint plus noise. To observe the effect of Peiyuan Jieyu recipe on body temperature, blepharoptosis and loop rate of reserpine depression model mice, and on the immobility time of forced swimming in acute stress model and chronic stress model mice. The contents of 5-hydroxytryptamine (5-HT), norepinephrine (NA) and indoleamine 3-dioxygenase (IDO) in brain of acute stress model mice were detected by enzyme-linked immunosorbent assay (ELISA). Fluorescence quantitative PCR was used to detect the expression of IDO mRNA in the brain of chronic emergency model mice. Results Peiyuan Jieyu prescription could antagonize the hypothermia of reserpine depression model mice (P0.01). In addition, high dose could also improve the condition of eyelid droop and motor failure induced by reserpine (P0.05), while the middle dose group could improve the condition of motility (P0.05). Peiyuan Jieyu recipe could shorten the immobility time (P0.05U P0.01) and significantly increase the content of 5-HTNNA in the brain (P0.05U P0.01) in the low dose group. Peiyuan Jieyu prescription could shorten the immobility time of forced swimming in chronic stress model mice, increase the content of 5-HT in brain tissue, decrease the content of IDO and up-regulate the expression of IDO mRNA (P0.05). Conclusion Peiyuan Jieyu prescription can ameliorate the depression symptoms of mice caused by reserpine, forced swimming and chronic stress. The mechanism may be related to the increase of monoamine neurotransmitter 5-HT na, the inhibition of IDO activity and the down-regulation of IDO mRNA expression in the brain of depression model mice.
【作者单位】：北京中医药大学中药学院;北京中医药大学中医学院;
【基金】：国家自然科学基金资助项目(No.81373584)~~
【分类号】：R285.5;R-332

【相似文献】