FGL2在泡球蚴感染所致DC细胞成熟中的作用研究
发布时间：2018-09-09 20:39
【摘要】：目的研究FGL2在泡球蚴感染所致DC细胞成熟中的作用及意义。方法 8~10周龄雌性野生型BALB/c小鼠12只和FGL2基因敲除小鼠12只,均随机分为2组:感染组6只,对照组6只。实验组腹腔注射0.1ml泡球蚴混悬液,对照组于相同部位注射等量PBS。感染后4个月将小鼠处死,取脾,制备脾细胞悬液,采用流式细胞术检测DC细胞成熟表面标记CD80及CD86的表达。留取血清,采用ELISA测定FG12水平。结果泡球蚴载量感染晚期fgl2-/-小鼠低于野生型小鼠,(F=8.426,P0.05)。qRT-PCR检测泡球蚴感染晚期Em14-3-3mRNA表达,fgl2-/-小鼠低于野生型小鼠,(F=16.070,P0.05);ELISA检测泡球蚴感染早期野生型小鼠和对照组血清FGL2,感染组高于对照组(F=13.995,P0.05);流式细胞术检测泡球蚴感染小鼠腹腔细胞和脾脏细胞中CD11b+DC和CD11c+DC细胞CD80表达水平发现前者泡球蚴感染fgl2-/-小鼠晚期腹腔细胞中CD80表达水平显著高于野生型小鼠(F=236.303,P0.05),后者在泡球蚴感染fgl2-/-小鼠晚期腹腔细胞和脾脏细胞CD80的表达水平均显著高于野生型小鼠(F=35.096,P0.05);ELISA检测泡球蚴感染鼠中血清TNF-α水平发现泡球蚴感染fgl2-/-小鼠表达高于野生型小鼠(F=13.612,P0.05)。结论在泡球蚴感染小鼠晚期,FGL2高表达可能参与泡球蚴感染引起的DC细胞成熟度降低及由此所致的免疫逃避有关。
[Abstract]:Objective to study the role and significance of FGL2 in DC cell maturation induced by alveolar hydatid infection. Methods 12 female wild-type BALB/c mice and 12 FGL2 knockout mice were randomly divided into two groups: infection group (n = 6) and control group (n = 6). The experimental group was intraperitoneally injected with 0.1ml alveolar hydatid suspension, while the control group was injected with the same amount of PBS. at the same site. Four months after infection, mice were killed and spleen cells were taken to prepare spleen cell suspensions. Flow cytometry was used to detect the expression of CD80 and CD86 on the mature surface of DC cells. The serum was collected and the level of FG12 was determined by ELISA. Results the load of alveolar hydatid in late stage fgl2-/- mice was lower than that in wild type mice. (FG 8.426 P 0.05). QRT-PCR was used to detect the expression of Em14-3-3mRNA in late stage of alveolar hydatid infection. The expression of Em14-3-3mRNA in mice with late infection was lower than that in wild type mice. (FF16. 070%) Elisa was high in early stage wild-type mice infected with alveolar echinococci and control group in serum FGL2, infection. The expression level of CD11b DC and CD11c DC cells in peritoneal cells and spleen cells of mice infected with alveolar echinococcosis was detected by flow cytometry. It was found that the expression of CD80 in the late stage of fgl2-/- mice infected with alveolar hydatid was significantly higher than that in mice infected with wild field hydatid. The expression of CD80 in the late stage of fgl2-/- mice infected with alveolar hydatid was significantly higher than that in wild-type mice (FF35.096P05). The expression of TNF- 伪 in serum of mice infected with alveolar echinococci was detected by Elisa. The expression of TNF- 伪 in mice infected with alveolar echinococci was found to be higher than that in mice infected with fgl2-/-. It was higher than that of wild type mice (FG 13.612 P 0.05). Conclusion the high expression of FGL2 in the late stage of alveolar hydatid infection may be involved in the decrease of DC cell maturity caused by alveolar hydatid infection and the immune evasion caused by it.
【作者单位】： 新疆医科大学基础医学院组织学与胚胎学教研室;新疆医科大学第一附属医院新疆包虫病基础医学重点实验室;
【基金】：新疆维吾尔自治区自然科学基金面上项目(No.2014211C035)
【分类号】：R383.3
本文编号：2233506
[Abstract]:Objective to study the role and significance of FGL2 in DC cell maturation induced by alveolar hydatid infection. Methods 12 female wild-type BALB/c mice and 12 FGL2 knockout mice were randomly divided into two groups: infection group (n = 6) and control group (n = 6). The experimental group was intraperitoneally injected with 0.1ml alveolar hydatid suspension, while the control group was injected with the same amount of PBS. at the same site. Four months after infection, mice were killed and spleen cells were taken to prepare spleen cell suspensions. Flow cytometry was used to detect the expression of CD80 and CD86 on the mature surface of DC cells. The serum was collected and the level of FG12 was determined by ELISA. Results the load of alveolar hydatid in late stage fgl2-/- mice was lower than that in wild type mice. (FG 8.426 P 0.05). QRT-PCR was used to detect the expression of Em14-3-3mRNA in late stage of alveolar hydatid infection. The expression of Em14-3-3mRNA in mice with late infection was lower than that in wild type mice. (FF16. 070%) Elisa was high in early stage wild-type mice infected with alveolar echinococci and control group in serum FGL2, infection. The expression level of CD11b DC and CD11c DC cells in peritoneal cells and spleen cells of mice infected with alveolar echinococcosis was detected by flow cytometry. It was found that the expression of CD80 in the late stage of fgl2-/- mice infected with alveolar hydatid was significantly higher than that in mice infected with wild field hydatid. The expression of CD80 in the late stage of fgl2-/- mice infected with alveolar hydatid was significantly higher than that in wild-type mice (FF35.096P05). The expression of TNF- 伪 in serum of mice infected with alveolar echinococci was detected by Elisa. The expression of TNF- 伪 in mice infected with alveolar echinococci was found to be higher than that in mice infected with fgl2-/-. It was higher than that of wild type mice (FG 13.612 P 0.05). Conclusion the high expression of FGL2 in the late stage of alveolar hydatid infection may be involved in the decrease of DC cell maturity caused by alveolar hydatid infection and the immune evasion caused by it.
【作者单位】： 新疆医科大学基础医学院组织学与胚胎学教研室;新疆医科大学第一附属医院新疆包虫病基础医学重点实验室;
【基金】：新疆维吾尔自治区自然科学基金面上项目(No.2014211C035)
【分类号】：R383.3
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