43株甘肃临床分离非结核分枝杆菌分类鉴定

发布时间：2018-09-10 15:00

【摘要】：目的了解甘肃当前临床流行的非结核分枝杆菌(NTM)的病原谱特点及其主要NTM种类,为指导临床诊疗、有效防治NTM肺病提供参考依据。方法收集2012年~2014年来源于甘肃省不同地区的875株临床分离分枝杆菌菌株,经PNB/TCH方法初步鉴定为疑似NTM菌株,采用16SrRNA基因测序技术进行菌种鉴定。结果 875株分枝杆菌临床分离菌株中分离出疑似NTM菌株46株。经16SrRNA基因序列分析鉴定,43株为NTM,3株为诺卡氏菌。43株NTM菌株分别为胞内分枝杆菌、堪萨斯分枝杆菌、塞内加尔分枝杆菌、鸟分枝杆菌、戈登氏分枝杆菌、楚尔盖分枝杆菌、罕见分枝杆菌、偶然分枝杆菌和脓肿分枝杆菌。其中,胞内分枝杆菌有31株,占总NTM菌株数的72.09%。结论甘肃省非结核分枝杆菌群以胞内分枝杆菌为主,应用16SrRNA基因序列分析鉴定方法能准确鉴定出NTM菌种,为临床诊治提供依据。
[Abstract]:Objective to investigate the pathogenetic characteristics and main NTM types of non-tuberculous bacilli (NTM) in Gansu province, and to provide reference for clinical diagnosis and treatment and effective prevention and treatment of NTM pulmonary disease. Methods A total of 875 clinical isolates of Mycobacterium were collected from different regions of Gansu Province from 2012 to 2014. They were identified as suspected NTM strains by PNB/TCH method and identified by 16SrRNA gene sequencing technique. Results 46 suspected NTM strains were isolated from 875 clinical isolates of Mycobacterium. By 16SrRNA gene sequence analysis, 43 NTM,3 strains were identified as Nocka's bacilli. 43 NTM strains were identified as Mycobacterium endocellularis, Mycobacterium Kansas, Mycobacterium Senegalese, Mycobacterium birdis, Mycobacterium Gordon, Mycobacterium chuergai, respectively. Rare Mycobacterium, Mycobacterium fortuitum, and Mycobacterium abscess. Among them, there were 31 strains of Mycobacterium endocytogenes, accounting for 72.09% of the total number of NTM strains. Conclusion Mycobacterium endocytogenes is the main group of non-tuberculous mycobacteria in Gansu province. NTM strains can be identified accurately by using 16SrRNA gene sequence analysis method, which provides a basis for clinical diagnosis and treatment.
【作者单位】：甘肃省兰州市肺科医院;
【基金】：兰州市科技计划项目(No.2014-ZD-01)资助~~
【分类号】：R378.911

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