肾虚型老年痴呆动物模型的建立

发布时间：2018-09-11 12:03

【摘要】：目的探讨通过皮下注射氢化可的松复合侧脑室注射β-淀粉样蛋白(Aβ)建立肾虚型老年痴呆动物模型的方法,为抗痴呆中药新药的研究提供实验模型。方法分别给予动物皮下注射氢化可的松、侧脑室注射β-淀粉样蛋白25-35(Aβ25-35)、皮下注射氢化可的松复合侧脑室注射Aβ25-35,通过体质量变化、跑步力竭时间、免疫学指标、血清皮质酮水平变化评价是否造成动物肾虚,通过morris水迷宫测试、脑组织病理学变化评价是否造成痴呆。结果与对照组相比,氢化可的松组动物出现体质量增长缓慢,自主活动减少,水迷宫上台潜伏期稍有延长,跑步力竭时间缩短,胸腺和脾指数降低,脾细胞刺激指数降低,血清皮质酮值降低;脑室注射Aβ的动物水迷宫上台潜伏期延长,但不出现前述的一系列肾虚表现;皮下注射氢化可的松复合侧脑室注射Aβ6μg的动物既出现肾虚的表现又出现学习记忆障碍;皮下注射氢化可的松复合侧脑室注射Aβ3μg也造成动物的肾虚表现,但并未造成严重的学习记忆障碍。结论皮下注射氢化可的松造成动物肾虚表现,侧脑室注射Aβ25-356μg可造成动物学习记忆障碍,皮下注射氢化可的松复合侧脑室注射Aβ25-356μg的动物既有肾虚证的表现也有学习记忆障碍,认为造成了肾虚型老年痴呆模型。
[Abstract]:Objective to establish an animal model of senile dementia with kidney deficiency by subcutaneous injection of hydrocortisone and 尾 -amyloid protein (A 尾). Methods the animals were given hydrocortisone subcutaneously, 尾 -amyloid 25-35 (A 尾 25-35) was injected into lateral ventricle and A 尾 25-35 was injected subcutaneously by hydrocortisone combined with A 尾 25-35 in lateral ventricle. The changes of serum corticosterone level were used to evaluate whether the kidney deficiency was caused in animals, and whether dementia was caused by morris water maze test and brain histopathological changes. Results compared with the control group, hydrocortisone group showed slow growth of body mass, decreased spontaneous activity, slightly prolonged stage latency of water maze, shortened running exhaustion time, decreased thymus and spleen index, and decreased spleen cell irritation index. The serum corticosterone level was decreased, the latency of water labyrinth was prolonged after intraventricular injection of A 尾, but there was not a series of kidney deficiency symptoms mentioned above. Hypodermic injection of hydrocortisone and injection of A 尾 6 渭 g into lateral ventricle showed both kidney deficiency and learning and memory disorder, and hypodermic injection of hydrocortisone combined with A 尾 3 渭 g also resulted in kidney deficiency. But it did not cause severe learning and memory impairment. Conclusion Hypodermic injection of hydrocortisone can cause kidney deficiency in animals, and intracerebroventricular injection of A 尾 25-356 渭 g can cause learning and memory impairment in animals. Subcutaneous injection of hydrocortisone combined with A 尾 25-356 渭 g injection of A 尾 25-356 渭 g has both kidney deficiency and learning and memory impairment. It is believed that the model of senile dementia with kidney deficiency is caused.
【作者单位】：中国医学科学院北京协和医学院药用植物研究所;
【基金】：国家科技重大新药创制项目(2009ZX09502-017)
【分类号】：R-332

【参考文献】