两种2型糖尿病小鼠溃疡创面愈合比较

发布时间：2018-09-17 08:50

【摘要】：目的比较目前常用的基因自发突变2型糖尿病小鼠C57BL/6J-db/db和STZ诱导的C57BL/6J小鼠的溃疡创面愈合特征,为应用稳定的糖尿病小鼠溃疡模型进行相关动物实验研究提供依据。方法建立糖尿病小鼠溃疡模型,统计0、3、5、7、10、14 d小鼠创面动态愈合率及愈合时间;7、14 d取组织,HE染色和Masson染色,免疫组化(CD31和PCNA)观察创面病理组织变化;荧光定量测定愈合相关因子collagen-IIIα、α-SMA的基因表达。结果基因突变db/db小鼠较STZ诱导小鼠的创面愈合时间显著延迟,由(16.6±0.8)d延长至(20.2±1.3)d(P0.001);db/db组较STZ组肉芽组织生长缓慢,新生上皮长度不足、胶原沉积紊乱,创面愈合较差;7 d,db/db组的CD31和PCNA显著性低表达(P0.01);7、14 d,db/db组基因上调量的倍数明显低于STZ组。结论两种糖尿病小鼠创面均出现愈合延迟,但基因突变糖尿病db/db小鼠相比于STZ诱导组小鼠在创面愈合延迟的程度上和愈合难易程度上,更适合进行糖尿病溃疡创面的研究。
[Abstract]:Objective to compare the wound healing characteristics of C57BL/6J mice induced by C57BL/6J-db/db and STZ in type 2 diabetic mice with gene spontaneous mutation. Methods the ulcer model of diabetic mice was established. The dynamic wound healing rate and healing time were calculated on day 1014. The tissue was stained with HE and Masson. The pathological changes of wound were observed by immunohistochemistry (CD31 and PCNA). The gene expression of collagen-III 伪, 伪 -SMA was measured quantitatively. Results the wound healing time of db/db mice induced by STZ was significantly delayed than that of STZ mice. The wound healing time was prolonged from (16.6 卤0.8) days to (20.2 卤1.3) d (P0.001) d in db/db mice, and the granulation tissue grew slower, the length of neonate epithelium was insufficient and collagen deposition was disorder in STZ group than in STZ group. The expression of CD31 and PCNA was significantly lower (P0.01) in the group of poor wound healing at 7 days than in the group of STZ on the 7th day after treatment (P0.01), and the multiple of up-regulation of the gene was significantly lower in the group of STZ than that in the group of STZ. Conclusion both diabetic mice have delayed wound healing, but db/db mice with gene mutation are more suitable for the study of diabetic ulcer wounds than those in STZ induced mice.
【作者单位】：广东药科大学基础学院药用生物活性物质研究所;广州军区广州总医院;广东药科大学生命科学与生物制药学院;
【基金】：国家自然科学基金项目(81502520)
【分类号】：R-332;R587.2

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