不同高压氧预处理对大鼠肾缺血再灌注损伤的研究

发布时间：2018-10-08 07:47

【摘要】：目的研究不同时间高压氧预处理对大鼠肾脏缺血再灌注损伤的影响并探讨其机制。方法雌性SD大鼠50只随机分为5组:假手术组(S组,n=10)打开腹腔不夹闭肾蒂;缺血再灌注组(IR组,n=10)夹闭双侧肾蒂30 m in后再灌注;高压氧预处理1组(H1,n=10);高压氧预处理2组(H2,n=10);高压氧预处理3组(H3,n=10),分别接受连续高压氧4、8、16 d治疗。高压氧结束后24 h,行肾脏缺血再灌注手术。每组大鼠术后24 h后处死,检测血尿素氮(BUN)、肌酐(Cr)、丙二醛(m alon ic d ialde-hyde,MDA),肿瘤坏死因子-α(tumor necrosis factor-,αTNF-α)、白介素-6(interleuk in-6,IL-6)及肾脏病理检查。结果 IR组BUN、Cr、MDA、TNF-α、IL-6高于S组(P0.01),IR组肾小管上皮细胞均有不同程度的坏死,间质水肿,大量炎细胞浸润,血管渗透性增加,肾小球萎缩;H1、H2、H3组BUN、Cr、MDA、TNF-α、IL-6均低于IR组(P0.05),H1、H2、H3组肾脏仅有肾小管上皮细胞肿胀,少量炎症细胞浸润;H3组TNF-α、IL-6、MDA高于H2组(P0.05)。结论连续高压氧4、8和16 d预处理均能减轻大鼠肾脏缺血再灌注损伤;连续16 d高压氧预处理作用较8 d作用减弱。
[Abstract]:Objective to study the effects of hyperbaric oxygen preconditioning on renal ischemia reperfusion injury in rats and its mechanism. Methods Fifty female SD rats were randomly divided into 5 groups: sham operation group (S group) without intraperitoneal clamping, ischemia reperfusion group (IR group) with bilateral renal pedicle occlusion for 30 m in and reperfusion group (IR group). Group 1 was pretreated with hyperbaric oxygen (H1), group 2 with hyperbaric oxygen preconditioning (group 2), group 3 with hyperbaric oxygen preconditioning (group 3) were treated with continuous hyperbaric oxygen (HBO) for 8 days for 16 days. Renal ischemia reperfusion was performed 24 hours after hyperbaric oxygen. The rats in each group were killed 24 hours after operation. Blood urea nitrogen (BUN),) creatinine (Cr), malondialdehyde (m alon ic d ialde-hyde,MDA), tumor necrosis factor- 伪 (tumor necrosis factor-, 伪 TNF- 伪 (TNF- 伪), interleukin-6 (interleuk in-6,IL-6) and renal pathology were detected. Results BUN,Cr,MDA,TNF- 伪 IL-6 in IR group was higher than that in S group (P0.01). In IR group, renal tubular epithelial cells were necrosis, interstitial edema, inflammatory cell infiltration and increased vascular permeability. The levels of BUN,Cr,MDA,TNF- 伪 and IL-6 in glomerular atrophy group were lower than those in IR group (P0.05). The renal tubule epithelial cells in H3 group were only swollen, and a few inflammatory cells infiltrated in H3 group were higher than that in H2 group (P0.05). Conclusion continuous hyperbaric oxygen preconditioning for 4 days and 16 days can attenuate renal ischemia-reperfusion injury in rats, and the effect of hyperbaric oxygen preconditioning for 16 days is weaker than that for 8 days.
【作者单位】：安徽医科大学附属省立医院ICU;
【分类号】：R363

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