IFN-α通过IFI16抑制人脑血管外膜成纤维细胞增殖并促进细胞凋亡

发布时间：2018-10-11 10:15

【摘要】：目的:探讨干扰素-α(IFN-α)是否通过诱导干扰素诱导蛋白16(IFI16)表达抑制人脑血管外膜成纤维细胞(HBVAFs)增殖与促进细胞凋亡。方法:应用转染针对IFI16基因的小干扰RNA(siRNA)和/或IFN-α瞬时干预体外培养的HBVAFs。通过蛋白免疫印迹(Western blot)法和Real-time PCR法测定细胞中IFI16、P53、P21蛋白和mRNA表达水平的变化。用MTT法检测细胞活力,流式细胞术测定细胞周期及凋亡。结果:与未干预组比较,IFN-α(2 000~5 000 kU/L)干预HBVAFs后,IFI16蛋白表达水平明显上调,但不同浓度IFN-α组之间无统计学差异。使用2 000 kU/L IFN-α可诱导HBVAFs中P53及P21蛋白和mRNA表达水平上调,同时抑制细胞增殖与促进细胞凋亡。但在转染了IFI16 siRNA的HBVAFs中IFN-α的上述作用受到了抑制。结论:IFN-α抑制HBVAFs增殖与促进其凋亡的作用可能部分与其诱导IFI16表达有关。
[Abstract]:Aim: to investigate whether interferon- 伪 (IFN- 伪) induces the expression of interferon-inducible protein 16 (IFI16) to inhibit the proliferation of human cerebrovascular adventitia fibroblasts (HBVAFs) and promote apoptosis. Methods: small interfering RNA (siRNA) transfected with IFI16 gene and / or IFN- 伪 were used to interfere with HBVAFs. in vitro. The expression of IFI16,P53,P21 protein and mRNA were detected by Western blot (Western blot) and Real-time PCR. MTT assay was used to detect cell viability, and flow cytometry was used to detect cell cycle and apoptosis. Results: compared with the control group, the expression of IFI16 protein increased significantly after IFN- 伪 (2 000 ~ 5 000 kU/L) intervention in HBVAFs, but there was no significant difference between the different concentration of IFN- 伪 groups. The expression of p53 and P21 protein and mRNA in HBVAFs was up-regulated by 2 000 kU/L IFN- 伪, and the proliferation of cells was inhibited and apoptosis was promoted at the same time. However, the above effects of IFN- 伪 in HBVAFs transfected with IFI16 siRNA were inhibited. Conclusion: the inhibitory effect of IFN- 伪 on the proliferation and apoptosis of HBVAFs may be partly related to its induction of IFI16 expression.
【作者单位】：贵州省人民医院心内科;
【基金】：国家自然科学基金项目(No.81260030)
【分类号】：R363

【相似文献】